Anticancer Activity of Bacteriophage T4 and its Mutant HAP1 in Mouse Experimental Tumour Models

Background: Previously, we have shown the ability of the bacteriophage T4 and its substrain HAP1 (selected for a higher affinity to melanoma cells) to reveal antimetastatic activity in a mouse melanoma model. Here, we investigated the potential phage anticancer activity in primary tumour models. Mat...

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Published inAnticancer research Vol. 24; no. 6; pp. 3991 - 3995
Main Authors Dabrowska, Krystyna, Opolski, Adam, Wietrzyk, Joanna, Switala-Jelen, Kinga, Godlewska, Joanna, Boratynski, Janusz, Syper, Danuta, Weber-Dabrowska, Beata, Gorski, Andrzej
Format Journal Article
LanguageEnglish
Published Attiki International Institute of Anticancer Research 01.11.2004
Subjects
Animals
Bacteria
Bacteriophage T4 - genetics
Bacteriophage T4 - physiology
Biological and medical sciences
Carcinoma, Lewis Lung - therapy
Carcinoma, Lewis Lung - virology
Female
Medical sciences
Melanoma, Experimental - therapy
Melanoma, Experimental - virology
Mice
Mice, Inbred C57BL
Mutation
Tumors
Antineoplastic agent
Bacteriophages
Animal model
Rodentia
Activity
melanoma
purified bacteriophages
Vertebrata
Mammalia
Cancerology
Mouse
Tumor
Mutation
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Summary:Background: Previously, we have shown the ability of the bacteriophage T4 and its substrain HAP1 (selected for a higher affinity to melanoma cells) to reveal antimetastatic activity in a mouse melanoma model. Here, we investigated the potential phage anticancer activity in primary tumour models. Materials and Methods: Mice were inoculated subcutaneously with B16 or LLC cells (collected from in vitro culture). Bacteriophages T4 and HAP1 were injected intraperitoneally daily (8×10 8 pfu/mouse, except the experiment concerning the dose-dependence). Results: Treatment with purified preparations of bacteriophage T4 resulted in significant reduction of tumour size, the effect being dose-dependent. HAP1 was more effective than T4 and its activity was also dose-dependent. Parallel experiments with non-purified bacteriophage lysates resulted in significant stimulation of tumour growth. Conclusion: These data suggest that purified bacteriophages may inhibit tumour growth, a phenomenon with potentially important clinical implications in oncology.
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ISSN:0250-7005
1791-7530