Absence of Oncogenic Mutations of RAS Family Genes in Soft Tissue Sarcomas of 100 Japanese Patients

• Published in: Anticancer research Vol. 30; no. 1; pp. 245 - 252
• Main Authors: YONGHUI JIN, SHIMA, Yasuko, FURU, Moritoshi, AOYAMA, Tomoki, NAKAMATA, Takeharu, NAKAYAMA, Tomitaka, NAKAMURA, Takashi, TOGUCHIDA, Junya
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.01.2010
• Subjects: Adolescent; Adult; Aged; Aged, 80 and over; Biological and medical sciences; Cell Line, Tumor; Child; Dermatology; DNA Mutational Analysis; Female; Genes, ras; Humans; Japan; Male; Medical sciences; Middle Aged; Polymerase Chain Reaction; Polymorphism, Single-Stranded Conformational; Sarcoma - genetics; Tumors; Tumors of the skin and soft tissue. Premalignant lesions; Young Adult; Asia; Japan; Human; RAS; Japan; Soft tissue sarcoma; Malignant tumor; Carcinogenesis; Cancerology; C-Onc gene; Genetics; Multigene family; Mutation; Ras gene; Protooncogene; Cancer
• ISSN: 0250-7005; 1791-7530

Background: Activating point mutations of genes of the RAS family (KRAS, HRAS and NRAS genes) are frequently found in carcinomas, but their prevalence in sarcomas varies considerably among ethnic groups. No extensive studies in Japanese patients have been performed. Materials and Methods: Mutation analyses of three RAS genes (KRAS, HRAS and NRAS) were performed using polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analyses and PCR direct sequencing in one hundred cases of soft tissue sarcoma (STS) as well as six STS cell lines from Japanese patients. Results: No mutations were found in two hot spot regions (codon 12-13 and 61) of the three RAS genes. Conclusion: Activating mutations of the RAS gene family are uncommon events in soft tissue sarcomas in Japanese patients.