p53 Intronic G13964C Variant in Colon Cancer and its Association with HPV

• Published in: Anticancer research Vol. 25; no. 4; pp. 2767 - 2769
• Main Authors: BUYRU, Nur, TEZOL, Ayda, DALAY, Nejat
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.07.2005
• Subjects: Alleles; Biological and medical sciences; Case-Control Studies; Colonic Neoplasms - genetics; Colonic Neoplasms - virology; Gastroenterology. Liver. Pancreas. Abdomen; Genes, p53 - genetics; Humans; Introns; Medical sciences; Papillomaviridae; Papillomavirus Infections - complications; Polymerase Chain Reaction; Polymorphism, Restriction Fragment Length; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Tumors; Papovaviridae; Malignant tumor; intron 6; p53; Papillomavirus; Virus; Variant; Human papillomavirus; Colon cancer; Cancerology; TP53 Gene; Intron; Digestive diseases; Intestinal disease; Tumor suppressor gene
• ISSN: 0250-7005; 1791-7530

Background: Inactivation of p53 is the most common change identified in human cancer. Nucleotide alterations in p53 intron 6 have been reported to be associated with the dysregulation of p53 function and tumor development. The aim of our study was to assess whether the intron 6 G13964C variant of the p53 gene is associated with the presence of human papillomavirus (HPV) as a risk factor in colon cancer. Materials and Methods: To determine whether the intronic G13964C alteration is involved in colon carcinogenesis, surgical specimens from 55 patients with colon cancer and HPV-positive tumors were examined by PCR-RFLP. Blood samples from 77 healthy subjects were used as the control group. Results: The 13964C variant was found in 14.5% of colon cancer patients and 12.9% of the control subjects. One patient and two controls were homozygous for this base change. Conclusion: No evidence of a significant association between the p53 G13964C allele and colon carcinoma was found.