Deficiency of queuine, a highly modified purine base, in transfer RNAs from primary and metastatic ovarian malignant tumors in women

The tRNAs from rapidly growing tissues, particularly from neoplasia, often exhibit queuine deficiency. In order to check whether different kinds of ovarian tumors display queuine deficiencies we have analyzed tRNA samples from 16 ovarian malignancies. The tRNAs from histologically normal myometrium...

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Published inCancer research (Chicago, Ill.) Vol. 54; no. 16; pp. 4468 - 4471
Main Authors BARANOWSKI, W, DIRHEIMER, G, JAKOWICKI, J. A, KEITH, G
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 15.08.1994
Subjects
Adolescent
Adult
Biological and medical sciences
Female
Female genital diseases
Guanine - analogs & derivatives
Guanine - analysis
Gynecology. Andrology. Obstetrics
Humans
Medical sciences
Middle Aged
Ovarian Neoplasms - chemistry
Ovarian Neoplasms - pathology
RNA, Neoplasm - chemistry
RNA, Transfer - chemistry
Tumors
Human
Prognosis
tRNA
Malignant tumor
Female genital diseases
Ovarian diseases
Ovary
Tissue
Histological type
Female
Advanced stage
Molecular biology
Quantitative analysis
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Summary:The tRNAs from rapidly growing tissues, particularly from neoplasia, often exhibit queuine deficiency. In order to check whether different kinds of ovarian tumors display queuine deficiencies we have analyzed tRNA samples from 16 ovarian malignancies. The tRNAs from histologically normal myometrium (4 samples) and myoma (6 samples) were taken as healthy tissue and benign tumor references. Queuine deficiency was determined by an exchange assay using [8-3H]guanine and tRNA:guanine transglycosylase from Escherichia coli. The mean values of queuine deficiencies in tRNAs were: 10.95 +/- 2.21 (SD) pmol/A260 in gonadal and germ cell tumors (5 cases); 23.75 +/- 7.89 pmol/A260 in primary epithelial tumors (9 cases); and 34.58 +/- 7.18 pmol/A260 in metastatic tumors (2 cases). These values displayed statistically significant differences (P = 0.0003, Kruskal-Wallis test). The queuine deficiencies in tRNAs significantly increased when moving from well-differentiated through moderately differentiated to poorly differentiated tumors, with the highest values found in poorly differentiated metastatic tumors (P = 0.0002, Kruskal-Wallis test). Queuine deficiency determination in tRNAs is proposed as a factor for clinical outcome prognosis of ovarian malignancies.
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ISSN:0008-5472
1538-7445