Ubiquitin-ribosomal protein S27a gene overexpressed in human colorectal carcinoma is an early growth response gene

One of the extension proteins on the carboxy terminus of ubiquitin was reported as the ribosomal protein S27a. We have cloned a gene which encodes this ubiquitin hybrid protein from a complementary DNA library of a human colon carcinoma cell line. Northern blot analysis of surgical specimens from co...

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Published inCancer research (Chicago, Ill.) Vol. 53; no. 8; pp. 1916 - 1920
Main Authors JAU MIN WONG, MAFUNE, K.-I, YOW, H, RIVERS, E. N, RAVIKUMAR, T. S, STEELE, G. D, LAN BO CHEN
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 15.04.1993
Subjects
Animals
Base Sequence
Biological and medical sciences
Colonic Neoplasms - genetics
colorectal carcinoma
effects on
Gastroenterology. Liver. Pancreas. Abdomen
Gene Expression
Humans
man
Medical sciences
Metalloproteins
Molecular Sequence Data
Nuclear Proteins
phorbol 12-myristate 13-acetate diester
Proto-Oncogenes
Rats
ribosomal protein S27a
Ribosomal Proteins - genetics
RNA, Messenger - analysis
RNA-Binding Proteins
serum
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
transcription
Transcription, Genetic
Tumor Cells, Cultured
Tumors
ubiquitin
Ubiquitins - genetics
Human
Ubiquitin
Carcinoma
Nucleotide sequence
Pathogenesis
Hybrid
Malignant tumor
Gene expression
In vitro
Ribosomal protein
Rectum
Digestive diseases
Intestinal disease
Colon
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Summary:One of the extension proteins on the carboxy terminus of ubiquitin was reported as the ribosomal protein S27a. We have cloned a gene which encodes this ubiquitin hybrid protein from a complementary DNA library of a human colon carcinoma cell line. Northern blot analysis of surgical specimens from colon cancer patients showed that these messenger RNA levels were higher in tumor tissue than in adjacent normal mucosa. Furthermore, to investigate the role of this novel ubiquitin hybrid gene in cellular growth control, the responsiveness of this gene to serum growth factors was examined. Within 30 min after serum or 12-O-tetradecanoylphorbol-13-acetate stimulation, its messenger RNA expression in rat fibroblast cells (Rat 1) was increased. Nuclear runoff transcription studies showed that the kinetics of induction of this gene is almost identical to that of protooncogene c-jun or c-fos, the known early growth response genes. Thus, this ubiquitin hybrid gene appears to be a novel early growth response gene overexpressed in human colon cancer and warrants further studies in the pathogenesis of colorectal carcinoma.
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ISSN:0008-5472
1538-7445