Kappa opioid receptor-mediated regulation of dopaminergic neurons in the rat brain
The effect of the kappa opioid receptor agonist U-50,488 [trans-3,4-dichloro-N-methyl-N-[2-pyrrolindinyl)-cyclohexyl]-benze ne- acetamide] was examined on the activity of dopamine (DA) neurons comprising the nigrostriatal, mesolimbic, tuberoinfundibular and tuberohypophysial systems in the male rat...
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Published in | The Journal of pharmacology and experimental therapeutics Vol. 256; no. 2; pp. 500 - 505 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Pharmacology and Experimental Therapeutics
01.02.1991
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Subjects | |
Online Access | Get full text |
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Summary: | The effect of the kappa opioid receptor agonist U-50,488 [trans-3,4-dichloro-N-methyl-N-[2-pyrrolindinyl)-cyclohexyl]-benze
ne- acetamide] was examined on the activity of dopamine (DA) neurons comprising the nigrostriatal, mesolimbic, tuberoinfundibular
and tuberohypophysial systems in the male rat brain under basal and stimulated conditions. DA neuronal activity was estimated
by measuring: 1) the accumulation of 3,4-dihydroxyphenylalanine after administration of a decarboxylase inhibitor and 2) the
concentration of the DA metabolite 3,4-dihydroxyphenyla-cetic acid in brain (striatum, nucleus accumbens and median eminence)
and pituitary regions (intermediate lobe and neural lobe) containing terminals of these neurons. Under basal (non-stimulated)
conditions, U-50,488 produced a dose- and time-related decrease in the activity of tuberohypophysial DA neurons, but failed
to alter the activity of nigrostriatal, mesolimbic or tuberoinfundibular DA neurons. The ability of U-50,488 to inhibit the
basal activity of tuberohypophysial DA neurons was blocked by pretreatment with the kappa opioid antagonist nor-binaltorphimine.
The activity of nigrostriatal, mesolimbic and intermediate lobe tuberohypophysial DA neurons was increased by haloperidol,
whereas the activity of tuberoinfundibular DA neurons was increased by prolactin. U-50,488 decreased the activity of nigrostriatal,
mesolimbic and tuberohypophysial DA neurons in haloperidol-treated rats, and decreased the activity of tuberoinfundibular
DA neurons in prolactin-treated rats. These results indicate that activation of kappa opioid receptors inhibits all DA neuronal
systems in the brain, but that this effect on nigrostriatal, mesolimbic and tuberoinfundibular DA neurons is evident only
after these neurons have been activated. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0022-3565 1521-0103 |