Human prostate tissue antigens defined by murine monoclonal antibodies

• Published in: Cancer research (Chicago, Ill.) Vol. 46; no. 1; pp. 367 - 374
• Main Authors: STARLING, J. J, SIEG, S. M, BECKETT, M. L, WIRTH, P. R, WAHAB, Z, SCHELLHAMMER, P. F, LADAGA, L. E, POLESKIC, S, WRIGHT, G. L. JR
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 1986
• Subjects: Antibodies, Monoclonal - immunology; Antibody Specificity; Biological and medical sciences; Breast - immunology; Cell Line; Cell Membrane - immunology; Colon - immunology; Cross Reactions; Gynecology. Andrology. Obstetrics; Humans; Immunoenzyme Techniques; Kidney - immunology; Male; Male genital diseases; Medical sciences; Prostate - immunology; Prostatic Hyperplasia - immunology; Prostatic Neoplasms - immunology; Radioimmunoassay; Tumors; Human; Rodentia; Biological marker; Urogenital system diseases; Monoclonal antibody; Normal; Antigen; Vertebrata; Mammalia; Mouse; Tumor; Urogenital system; Prostate
• ISSN: 0008-5472; 1538-7445

BALB/c mice were hyperimmunized against a membrane preparation derived from a pool of transurethral resection specimens which included three benign prostatic hyperplasia and one prostate adenocarcinoma tissue samples. The activated lymphocytes were fused with the NS-1 mouse myeloma cell line, and supernatants from immunogen-reactive hybridomas were screened for antibody binding activity using a solid-phase radioimmunoassay against the Calu-1 human lung adenocarcinoma cell line and several membrane preparations derived from various normal human tissues. Hybridoma cultures secreting antibodies which did not appear cross-reactive were doubly cloned by limiting dilution and screened against a large panel of membrane preparations derived from normal prostate, benign prostatic hyperplasia, and prostate adenocarcinoma tissues as well as samples obtained from a variety of normal human tissues. The monoclonal antibodies were also evaluated against 24 normal, virally transformed, and malignant human cell lines. Two monoclonal antibodies were isolated which demonstrated a restricted binding activity to prostate antigens and were not widely cross-reactive with nonprostate normal tissues or cell lines. These antibodies were designated TURP-27 (IgG3, k) and TURP-73 (IgG2a, k). Both of these monoclonal antibodies were reactive against formalin-fixed, paraffin-embedded tissues in the immunoperoxidase assay and were subsequently tested against a variety of normal, hyperplastic, and malignant human tissues. These studies indicated that TURP-27 may be directed against a new prostate organ-associated marker and that TURP-73 is directed against an antigen expressed on prostate and a limited number of other tissues.