Gentamicin-induced alterations in pig kidney epithelial (LLC-PK1) cells in culture
The effect of gentamicin exposure was investigated in LLC-PK1 cells in culture. Gentamicin (0.5-2.0 mM) was added to the medium of cells which had been grown to confluency in the absence of antibiotics and antimycotics. Exposure to gentamicin (1-4 days) did not effect total cellular protein or DNA l...
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Published in | The Journal of pharmacology and experimental therapeutics Vol. 236; no. 1; pp. 254 - 262 |
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Main Authors | , , |
Format | Journal Article |
Language | English |
Published |
Bethesda, MD
American Society for Pharmacology and Experimental Therapeutics
01.01.1986
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Subjects | |
Online Access | Get full text |
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Summary: | The effect of gentamicin exposure was investigated in LLC-PK1 cells in culture. Gentamicin (0.5-2.0 mM) was added to the medium
of cells which had been grown to confluency in the absence of antibiotics and antimycotics. Exposure to gentamicin (1-4 days)
did not effect total cellular protein or DNA levels, total cell number or the release of various marker enzymes to the medium.
ATP levels in gentamicin-treated cells did not differ from control cells; however, medium from the gentamicin-treated cells
contained significantly lower lactic acid levels. Morphological examination by electron microscopy revealed gentamicin-elicited
myeloid body formation. Furthermore, total phospholipid level was elevated markedly in gentamicin-treated cells. Analysis
of specific phospholipid classes showed only phosphatidylcholine, phosphatidylinositol and polyphosphoinositide phospholipid
levels increased in a time-dependent manner. Phosphatidylinositol showed the highest percentage of increase. Raising the normal
medium calcium concentration (0.2 mg/ml) 1.5-, 2.0- or 3.0-fold did not alter gentamicin-induced elevation in cellular phosphatidylinositol
and phosphatidylcholine. Gentamicin exposure also resulted in a concentration-dependent increase in the turnover of LLC-PK1
cell-free fatty acids, monoglyceride, diglyceride and nonesterified cholesterol and a decrease in triglyceride turnover. Calcium
transport into and through the cell monolayer was inhibited markedly by gentamicin despite the fact that 45Ca++ binding to
gentamicin-treated cells was greater. These results demonstrate that manifestations of gentamicin toxicity in LLC-PK1 cells
parallel those reported in the whole animal thus making the LLC-PK1 cell in culture a valid system for elucidating the mechanism
of gentamicin-elicited alterations in renal epithelium. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 |
ISSN: | 0022-3565 1521-0103 |