Efficient Human Sperm Pronucleus Formation and Replication in Xenopus Egg Extracts

We have achieved efficient in vitro reactivation and replication of human sperm nuclei in frog egg extracts by constructing a 4-step protocol that mimics the events of fertilization and pronucleus formation in mammalian eggs. With use of this protocol, 78–97% of human sperm nuclei from fertile don...

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Published inBiology of reproduction Vol. 61; no. 4; pp. 912 - 920
Main Authors NEUBER, E, HAVARI, E, SANCHEZ, J. A, POWERS, R. D, WANGH, L. J
Format Journal Article
LanguageEnglish
Published Madison, WI Society for the Study of Reproduction 01.10.1999
Subjects
Animals
Biological and medical sciences
Cell Nucleus - physiology
DNA Replication
Female
Fertilization in Vitro - methods
Freshwater
Fundamental and applied biological sciences. Psychology
Humans
Male
Mammalian male genital system
Morphology. Physiology
Oocytes - cytology
Oocytes - physiology
Sperm-Ovum Interactions - physiology
Spermatozoa - cytology
Spermatozoa - physiology
Vertebrates: reproduction
Xenopus
Xenopus laevis
Human
Spermatozoa
Replication
DNA synthesis
Germinal cell
In vitro
Pronucleus
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Summary:We have achieved efficient in vitro reactivation and replication of human sperm nuclei in frog egg extracts by constructing a 4-step protocol that mimics the events of fertilization and pronucleus formation in mammalian eggs. With use of this protocol, 78–97% of human sperm nuclei from fertile donors synchronously swelled and completed full genome replication in about 2 h. We document the changes in nuclear structure that accompany efficient DNA synthesis and discuss future research and potential clinical implications of this new system.
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ISSN:0006-3363
1529-7268
DOI:10.1095/biolreprod61.4.912