Radiation-induced neoplastic transformation of human prostate epithelial cells

We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray do...

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Bibliographic Details
Published inCancer research (Chicago, Ill.) Vol. 56; no. 1; pp. 5 - 10
Main Authors KUETTEL, M. R, THRAVES, P. J, MIRA JUNG, VARGHESE, S. P, PRASAD, S. C, RHIM, J. S, DRITSCHILO, A
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 1996
Subjects
Animals
Biological and medical sciences
Carcinogenesis, carcinogens and anticarcinogens
Cell Transformation, Neoplastic - radiation effects
Cells, Cultured
Epithelium - metabolism
Epithelium - pathology
Epithelium - radiation effects
Humans
Karyotyping
Keratins - biosynthesis
Male
Medical sciences
Mice
Mice, Nude
Neoplasm Transplantation
Physical agents
Prostate - metabolism
Prostate - pathology
Prostate - radiation effects
Prostate-Specific Antigen - biosynthesis
Tumors
Chromosomal aberration
Adenocarcinoma
Human
Urinary system disease
Prostate disease
Toxicity
Rodentia
Tumorigenicity
Carcinogenesis
In vitro
In vivo
Vertebrata
Ionizing irradiation
Mammalia
Mouse
Animal
Malignant transformation
Established cell line
Abnormal chromosome
Epithelial cell
Male genital diseases
Prostate
Karyotype
Chromosome translocation
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Summary:We report the malignant transformation of human prostate epithelial cells (267B1) after multiple exposures to ionizing radiation. Carcinogenic progression of cells from immortal growth to anchorage-independent growth in soft agar to tumorigenicity in athymic mice resulted after a cumulative X-ray dose of 30 Gy. The tumors were characterized histologically as poorly differentiated adenocarcinomas, expressed prostate-specific antigen, and stained positive for keratin. No p53 or ras mutations were observed. Numerous chromosomal defects were noted on karyotypes after radiation exposure. However, chromosome 3 and 8 translocations were observed predominantly in the tumor outgrowths. These findings provide the first evidence of malignant transformation of human prostate epithelial cells exposed to ionizing radiation.
Bibliography:ObjectType-Article-2
SourceType-Scholarly Journals-1
ObjectType-Feature-1
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ISSN:0008-5472
1538-7445