Immunogenetic influence of Igh-1 phenotype on experimental herpes simplex virus type-1 corneal infection

• Published in: Investigative ophthalmology & visual science Vol. 29; no. 5; pp. 749 - 754
• Main Authors: Opremcak, EM, Wells, PA, Thompson, P, Daigle, JA, Rice, BA, Millin, JA, Foster, CS
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.05.1988; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Biological and medical sciences; Cornea - cytology; Cornea - microbiology; Experimental viral diseases and models; Female; Genes; Infectious diseases; Keratitis, Dendritic - genetics; Keratitis, Dendritic - immunology; Keratitis, Dendritic - microbiology; Male; Medical sciences; Mice; Mice, Inbred BALB C; Phenotype; Viral diseases; Virus Replication; Keratitis; Herpesviridae; Alphaherpesvirinae; Rodentia; Herpes; Infection; Virus; Vertebrata; Immunogenetics; Eye disease; Experimental disease; Mammalia; Mouse; Viral disease; Animal; Herpesvirus hominis
• ISSN: 0146-0404; 1552-5783

Patterns of herpes simplex virus type-1 (HSV-1) infection were studied in BALB/c congenic, Igh-1 disparate murine strains to establish the influence of Igh-1 phenotype on the development of keratopathy, trigeminal ganglionic latency and keratocyte permissivity. Eighty-two percent of C.AL-20 (Igh-1d) mice, 40% of BALB/cByJ (Igh-1a) mice and 12% of the C.B-17 (Igh-1b) mice developed herpes simplex keratitis (HSK) following corneal challenge with 2.5 X 10(4) PFU HSV-1 strain KOS. While disease frequency was directly proportional to HSV-1 challenge dose, relative resistance and susceptibility patterns in the congenic mice were constant and highly significant. F1 progeny from C.AL-20 X C.B-17 matings demonstrated the HSK pattern of the C.B-17 parent suggesting that Igh-1 linked resistance to HSK is dominantly inherited. Equivalent trigeminal ganglionic latency was established following ocular HSV-1 inoculation in the three congenic Igh-1 disparate murine strains. Cultured keratocytes from the three Igh-1 disparate murine strains demonstrated equivalent in vitro permissivity to HSV-1 replication. These data illustrate a strong correlation between Igh-1 phenotype and the development of a HSK in congenic mice. The susceptibility/resistance to HSK in these mice is unrelated to trigeminal ganglionic latency or keratocyte permissivity.