Xrcc-1 expression during male meiosis in the mouse

• Published in: Biology of reproduction Vol. 55; no. 3; pp. 630 - 635
• Main Authors: WALTER, C. A, TROLIAN, D. A, MCFARLAND, M. B, STREET, K. A, GURRAM, G. R, MCCARREY, J. R
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.09.1996
• Subjects: Aging - metabolism; Animals; Biological and medical sciences; Blotting, Northern; DNA - analysis; DNA Repair - physiology; DNA-Binding Proteins - biosynthesis; Fundamental and applied biological sciences. Psychology; In Vitro Techniques; Male; Mammalian male genital system; Meiosis - physiology; Mice; Mice, Inbred C57BL; Morphology. Physiology; Polymerase Chain Reaction; Ribonucleases - antagonists & inhibitors; Ribonucleases - metabolism; RNA - analysis; RNA - isolation & purification; Sertoli Cells - metabolism; Spermatogonia - metabolism; Testis - cytology; Testis - metabolism; Vertebrates: reproduction; X-ray Repair Cross Complementing Protein 1; Senescence; Spermatogenesis; Rodentia; Male; Testicle; Germinal cell; Gene expression; Male genital system; Vertebrata; Mammalia; Single strand break; Mouse; DNA; Mutation; Repair; Age
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod55.3.630

XRCC1 is involved in DNA strand-break repair, homologous recombination, and sister chromatid exchange and is expressed as a low-abundance mRNA with elevated expression in testis. The purpose of this study was to determine whether specific spermatogenic cell types have elevated Xrcc-1 expression and whether expression levels change in the testis with increased age. Northern blot analysis of mRNA prepared from testes of 15-, 25-, and 60-day-old mice revealed a single hybridizing band of 2.2 kb. Quantitative RNase protection assays revealed no changes in the level of Xrcc-1 expression in testis relative to DNA content among 6-, 12-, 18-, 24-, or 28-mo-old mice. Finally, reverse transcription coupled polymerase chain reaction amplification results demonstrated that Xrcc-1 expression is most abundant in pachytene spermatocytes and round spermatids with low expression in Sertoli cells, types A and B spermatogonia, preleptotene spermatocytes, and leptotene plus zygotene spermatocytes. The relatively abundant Xrcc-1 expression in pachytene spermatocytes and round spermatids suggests that Xrcc-1 is involved in DNA strand-break repair associated with meiotic recombination in addition to its previously implicated role in strand-break repair associated with base excision repair.