Hemagglutination and graft-versus-host disease in the severe combined immunodeficiency mouse lymphoproliferative disease model

In the course of evaluating the severe combined immunodeficiency mouse-human peripheral blood lymphocyte (SCID-PBL) model of lymphoproliferative disease, we noted hemagglutination occurring in peripheral blood smears of mice with serum human immunoglobulin levels greater than 1.0 mg/ml. The hemagglu...

Full description

Saved in:
Bibliographic Details
Published inThe American journal of pathology Vol. 140; no. 5; pp. 1187 - 1194
Main Authors Pirruccello, SJ, Nakamine, H, Beisel, KW, Kleveland, KL, Okano, M, Taguchi, Y, Davis, JR, Mahloch, ML, Purtilo, DT
Format Journal Article
LanguageEnglish
Published United States ASIP 01.05.1992
Subjects
Animals
Disease Models, Animal
Erythrocytes - metabolism
Flow Cytometry
Graft vs Host Disease - etiology
Graft vs Host Disease - pathology
Hemagglutination
Immunoglobulins - blood
Lymphoproliferative Disorders - blood
Lymphoproliferative Disorders - complications
Lymphoproliferative Disorders - pathology
Mice
Mice, SCID
Severe Combined Immunodeficiency - blood
Severe Combined Immunodeficiency - complications
Severe Combined Immunodeficiency - pathology
Online AccessGet full text

Cover

More Information
Summary:In the course of evaluating the severe combined immunodeficiency mouse-human peripheral blood lymphocyte (SCID-PBL) model of lymphoproliferative disease, we noted hemagglutination occurring in peripheral blood smears of mice with serum human immunoglobulin levels greater than 1.0 mg/ml. The hemagglutinating process was mediated by human anti-mouse red cell antibodies of the IgM class, peaked at five to seven weeks post-transfer of 5 to 7 x 10(7) human PBL and was generally self limiting. However, death resulted in some mice when serum immunoglobulin levels were greater than 3.0 mg/ml. The most severely affected mice had hemagglutination induced congestion of liver, lungs and spleen. Several mice also had lesions consistent with graft-versus-host disease (GVHD) including focal hepatic necrosis and destruction of mouse splenic hematopoietic elements. The lesions associated with hemagglutination and GVHD in SCID-PBL mice are distinct from those associated with EBV-induced lymphoproliferation. Recognition of these pathologic processes are required for a thorough understanding of the SCID-PBL model.
Bibliography:ObjectType-Article-1
SourceType-Scholarly Journals-1
ObjectType-Feature-2
content type line 23
ISSN:0002-9440
1525-2191