Immunocyte Ca2+Influx System Mediated by LTRPC2

• Published in: Science (American Association for the Advancement of Science) Vol. 293; no. 5533; pp. 1327 - 1330
• Main Authors: Sano, Yorikata, Inamura, Kohei, Miyake, Akira, Mochizuki, Shinobu, Yokoi, Hiromichi, Matsushime, Hitoshi, Furuichi, Kiyoshi
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 17.08.2001; American Association for the Advancement of Science
• Subjects: Adenosine Diphosphate Ribose - metabolism; Adenosine Diphosphate Ribose - pharmacology; Adenosine Triphosphate - metabolism; Adenosine Triphosphate - pharmacology; ADP-ribosyl Cyclase; ADP-ribosyl Cyclase 1; Antigens, CD; Antigens, Differentiation - metabolism; Apoptosis; Biological and medical sciences; Blood cells; Calcium - metabolism; Calcium Channels - metabolism; Cell Line; Cell lines; Cell physiology; Dialysis; Eosinophils - metabolism; Fundamental and applied biological sciences. Psychology; HEK293 cells; Humans; Immunocytes; Ion Channels; Jurkat Cells; Membrane and intracellular transports; Membrane Glycoproteins; Membrane Potentials; Membrane Proteins; Memory; Molecular and cellular biology; Monocytes - metabolism; NAD - metabolism; NAD - pharmacology; NAD+ Nucleosidase - metabolism; Neurons; Patch-Clamp Techniques; Pipettes; Reverse Transcriptase Polymerase Chain Reaction; T lymphocytes; T-Lymphocytes - metabolism; TRPM Cation Channels; Ion transport; Immunocyte; Activation; Uptake; T-Lymphocyte
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1062473

We characterized an activation mechanism of the human LTRPC2 protein, a member of the transient receptor potential family of ion channels, and demonstrated that LTRPC2 mediates Ca2+influx into immunocytes. Intracellular pyrimidine nucleotides, adenosine 5′-diphosphoribose (ADPR), and nicotinamide adenine dinucleotide (NAD), directly activated LTRPC2, which functioned as a Ca2+-permeable nonselective cation channel and enabled Ca2+influx into cells. This activation was suppressed by intracellular adenosine triphosphate. These results reveal that ADPR and NAD act as intracellular messengers and may have an important role in Ca2+influx by activating LTRPC2 in immunocytes.