An Oral Drug Delivery System Targeting Immune-Regulating Cells Ameliorates Mucosal Injury in Trinitrobenzene Sulfonic Acid-Induced Colitis
Control of immune-regulating cells in the colonic mucosa is important in the treatment of patients with inflammatory bowel disease (IBD). The aim of study was to examine the therapeutic effect of dexamethasone (DX) microspheres on 2,4,6-trinitrobenzene sulfonic acid (TNBS)-induced colitis in rats, a...
Saved in:
Published in | The Journal of pharmacology and experimental therapeutics Vol. 297; no. 3; p. 1122 |
---|---|
Main Authors | , , , , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
American Society for Pharmacology and Experimental Therapeutics
01.06.2001
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Control of immune-regulating cells in the colonic mucosa is important in the treatment of patients with inflammatory bowel
disease (IBD). The aim of study was to examine the therapeutic effect of dexamethasone (DX) microspheres on 2,4,6-trinitrobenzene
sulfonic acid (TNBS)-induced colitis in rats, a model for human Crohn's disease. DX microspheres and DX alone were administered
orally to rats with TNBS-induced colitis. The macroscopic score, histological score, myeloperoxidase (MPO) activity, nitric
oxide (NO) production, and gene expressions of proinflammatory cytokines, cyclooxygenase (COX)-1, and COX-2 in the colonic
tissue were determined. Proliferating cell nuclear antigen (PCNA) staining and expression of nuclear transcription factor
(NF)-κB in colonic tissues were also investigated. Macroscopic score, histological score, MPO activity, and NO production
in rats treated with DX microspheres were significantly lower than in those treated with DX alone. The gene expression of
proinflammatory cytokines and COX-2 in rats treated with DX microspheres was down-regulated, compared with that in rats treated
with DX alone. The number of PCNA-positive cells in the DX microsphere group was larger than in the group treated with DX
alone. DX microspheres suppressed NF-κB activation in TNBS-induced colitis more strongly than DX alone. Oral administration
of DX microspheres appears to ameliorate mucosal injury in TNBS-induced colitis. This drug delivery system could be an ideal
therapy for human IBD. |
---|---|
ISSN: | 0022-3565 1521-0103 |