Identification and physical mapping of genes expressed in the corpus luteum in cattle

• Published in: Animal genetics Vol. 34; no. 5; pp. 325 - 333
• Main Authors: Bønsdorff, T., Eggen, A., Gautier, M., Åsheim, H.-C., Rønningen, K., Lingaas, F., Olsaker, I.
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Science Ltd 01.10.2003; Wiley-Blackwell
• Subjects: Animals; Base Sequence; Blotting, Southern; cattle; Cattle - genetics; Chromosome Mapping; corpus luteum; Corpus Luteum - physiology; DNA Primers; DNA, Complementary - genetics; Expressed Sequence Tags; Female; Gene Expression; Life Sciences; Molecular Sequence Data; ovary; radiation hybrid panel; representational difference analysis; Reverse Transcriptase Polymerase Chain Reaction; Sequence Analysis, DNA; somatic cell hybrid panel; subtractive hybridization
• ISSN: 0268-9146; 1365-2052
• DOI: 10.1046/j.1365-2052.2003.01023.x

A representational difference analysis was performed to identify genes expressed in the corpus luteum of cattle. The corpus luteum is an ovarian structure that is essential for the establishment and maintenance of pregnancy. Knowledge of gene expression and function of corpus luteum will be important to improve fertility in humans and domestic animals. Housekeeping genes were removed from the corpus luteum representation (tester) using skeletal muscle as the subtracting agent (driver). A total of 80 clones of the final subtraction product were analysed by sequencing and 11 new bovine gene sequences were identified (pBTCL1-11). The sequences were mapped to segments of 10 different chromosomes using a somatic cell hybrid panel and a radiation hybrid panel. With one exception the locations are in agreement with published comparative maps of cattle and man. Expression in corpus luteum was verified by RT-PCR for all the 11 clones.