Antiproliferative Activity of Vitamin D Compounds in Combination with Cytostatics

• Published in: Anticancer research Vol. 26; no. 4A; pp. 2701 - 2705
• Main Authors: PELCZYNSKA, Marzena, SWITALSKA, Marta, MACIEJEWSKA, Magdalena, JAROSZEWICZ, Iwona, KUTNER, Andrzej, OPOLSKI, Adam
• Format: Journal Article Conference Proceeding
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.07.2006
• Subjects: Animals; Antineoplastic Combined Chemotherapy Protocols - pharmacology; BALB 3T3 Cells; Biological and medical sciences; Calcitriol - administration & dosage; Calcitriol - analogs & derivatives; Calcitriol - pharmacology; Cell Growth Processes - drug effects; Cell Line, Tumor; Cisplatin - administration & dosage; Dihydroxycholecalciferols - administration & dosage; Dihydroxycholecalciferols - pharmacology; Doxorubicin - administration & dosage; Drug Screening Assays, Antitumor; Humans; Medical sciences; Mice; Tumors; Antineoplastic agent; DNA topoisomerase (ATP-hydrolysing); Enzyme; Cholecalciferol(1,25-dihydroxy); Cytostatic; Calcitriol; Enzyme inhibitor; Malignant tumor; Doxorubicin; In vitro; Biological activity; Cisplatin; Alkylating agent; Isomerases; Cancerology; Vitamin D; Analog; Anthracyclins; Combined treatment; in vitro assay; vitamin D analogues; Platinum II Complexes; Cancer
• ISSN: 0250-7005

Calcitriol is a potent antiproliferative agent against various tumour cells in vitro. Here, the results of a study on vitamin D compounds (calcitriol's analogues PRI-1906 and PRI-2191) as potential agents in combined antitumour therapy in vitro are presented. Applying antiproliferative SRB and MTT assays, the growth inhibitory effects of the vitamin D compounds, applied alone or in combination with either cisplatin or doxorubicin, were measured. The following cancer cell lines were employed: A549 (human non-small cell lung carcinoma), B16 (murine melanoma), CCRF, HL-60 (human leukaemia), SW707 (human colon cancer), MCF-7, T47D (human breast cancer), WEHI-3 (mouse leukaemia) and normal cells: BALB 3T3 (normal murine fibroblast cell line). It was shown that the treatment of tumour cells, which are sensitive to vitamin D compounds, with the combination of vitamin D compounds and cytostatics decreased the inhibitory concentration 50% (IC 50 ) values compared with the effects of the cytostatics applied alone.