Medullary Thyroid Carcinoma: Autologous Tumor Cell Lines for Dendritic Cell Vaccination
Background: Medullary thyroid carcinoma (MTC) is a calcitonin-producing tumor of the parafollicular C-cells, accounting for 5-10% of all thyroid tumors. To date, the only effective treatment is the early and total surgical removal of all neoplastic tissue. As the prognosis of patients with advanced...
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Published in | Anticancer research Vol. 25; no. 6B; pp. 4225 - 4230 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Attiki
International Institute of Anticancer Research
01.11.2005
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Subjects | |
Online Access | Get full text |
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Summary: | Background: Medullary thyroid carcinoma (MTC) is a calcitonin-producing tumor of the parafollicular C-cells, accounting for
5-10% of all thyroid tumors. To date, the only effective treatment is the early and total surgical removal of all neoplastic
tissue. As the prognosis of patients with advanced MTC, unresectable or distant metastases is poor, and chemotherapy or irradiation
is of no significant value, alternative strategies have been sought. Materials and Methods: A promising treatment approach
for human MTC, that has already been introduced at our facility, is based on vaccination with autologous dendritic cells (DCs).
Strong evidence that vaccination with autologous tumor lysate-pulsed DCs induces a specific immune response in vivo has been
provided. However, the therapeutic success of this approach is sometimes critically limited by the small amount of tumor material
available, especially from patients operated at an early tumor stage. Thus, it would be to the best advantage to have sufficient
amounts of autologous tumor cells available for DC pulsing. Results: A method to generate viable autologous tumor cell cultures
from a variety of MTC tissue samples, even when the sample size is small, has been successfully established. These cell lines
maintain their neuroendocrine phenotype. In addition, it can be shown that these cells also display the biological features
of neuroendocrine tumor cells at the molecular level. Conclusion: The unlimited availability of these MTC cell lines makes
it possibler to specify cancerogenesis of MTC. In addition, the availability of sufficient amounts of tumor lysate from these
cell lines offers the advantage of prolonged immunotherapy. Finally, these cell lines could be elegantly used as read-out
system to monitor the in vivo immune response during immunotherapy with DC cell-based vaccination in patients suffering from
MTC. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0250-7005 1791-7530 |