Phylogenetic analysis of the small subunit ribosomal RNA of Marteilia refringens validates the existence of phylum Paramyxea (Desportes and Perkins, 1990)

Marteilia refringens is recognized as one of the most significant pathogens of bivalve molluscs. The nucleotide sequence of the small subunit ribosomal RNA gene of Marteilia refringens is used to elucidate the phylogenetic position of the phylum Paramyxea. Genomic DNA was extracted from sporangia of...

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Published inThe Journal of eukaryotic microbiology Vol. 47; no. 3; pp. 288 - 293
Main Authors BERTHE, F. C. J, LE ROUX, F, PEYRETAILLADE, E, PEYRET, P, RODRIGUEZ, D, GOUY, M, VIVARES, C. P
Format Journal Article
LanguageEnglish
Published Danvers, MA Blackwell 01.05.2000
Wiley
Subjects
Animals
Biological and medical sciences
Bivalvia - parasitology
Classification
Eukaryota - classification
Fundamental and applied biological sciences. Psychology
Life Sciences
Molecular Sequence Data
Ostreidae - parasitology
Other
Protozoa
RNA, Ribosomal - genetics
Sequence Homology, Nucleic Acid
Systematics. Geographical distribution. Morphology. Cytology
France
Europe
Protozoa
Host parasite relation
Apicomplexa
Genetic variability
Nucleotide sequence
Taxonomy
Mytilus edulis
Interspecific comparison
Chemotaxonomy
Parasite
Zoopathogen
Phylogeny
Subunit
Aquatic environment
Marteilia refringens
Bivalvia
Ribosomal RNA
Molecular probe
Invertebrata
Mollusca
Ostrea edulis
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Summary:Marteilia refringens is recognized as one of the most significant pathogens of bivalve molluscs. The nucleotide sequence of the small subunit ribosomal RNA gene of Marteilia refringens is used to elucidate the phylogenetic position of the phylum Paramyxea. Genomic DNA was extracted from sporangia of Marteilia, purified from infected blue mussels, Mytilus edulis, and flat oysters, Ostrea edulis. The sequences obtained from Marteilia species purified from both oysters and mussels were identical. The sequence identity was confirmed by in situ hybridization using a DNA probe targeted to a variable region of the ribosomal DNA. The small subunit ribosomal RNA gene sequence of M. refringens is very different from all known sequences of eukaryotic organisms, including those of myxosporeans and haplosporeans. Therefore, the phylum Paramyxea should continue to be recognized as an independent eukaryotic phylum.
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ISSN:1066-5234
1550-7408
DOI:10.1111/j.1550-7408.2000.tb00049.x