Inotodiol, a Lanostane Triterpenoid, from Inonotus obliquus Inhibits Cell Proliferation through Caspase-3-dependent Apoptosis
Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. Materials and Methods: Cell viability was measured by MTT assay. Casp...
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Published in | Anticancer research Vol. 28; no. 5A; pp. 2691 - 2696 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
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International Institute of Anticancer Research
01.09.2008
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Abstract | Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids
from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. Materials and Methods:
Cell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction.
The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF 1 mice. Results: The chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids
examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in
the P388 cells treated with inotodiol (30 μM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 μM) partially
inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg
inotodiol prolonged the number of survival days of the P388-bearing mice. Conclusion: Inotodiol inhibits cell proliferation
through apoptosis induction by activating caspase-3. |
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AbstractList | To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed.
Cell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction. The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF1 mice.
The chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in the P388 cells treated with inotodiol (30 microM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 microM) partially inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg inotodiol prolonged the number of survival days of the P388-bearing mice.
Inotodiol inhibits cell proliferation through apoptosis induction by activating caspase-3. BACKGROUNDTo investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed.MATERIALS AND METHODSCell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction. The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF1 mice.RESULTSThe chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in the P388 cells treated with inotodiol (30 microM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 microM) partially inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg inotodiol prolonged the number of survival days of the P388-bearing mice.CONCLUSIONInotodiol inhibits cell proliferation through apoptosis induction by activating caspase-3. Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. Materials and Methods: Cell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction. The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF 1 mice. Results: The chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in the P388 cells treated with inotodiol (30 μM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 μM) partially inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg inotodiol prolonged the number of survival days of the P388-bearing mice. Conclusion: Inotodiol inhibits cell proliferation through apoptosis induction by activating caspase-3. |
Author | MASAAKI NOMURA REIKO TANAKA TATSUO TAKAHASHI AIMI UESUGI SHINJIRO KOBAYASHI |
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Keywords | Cell proliferation Enzyme Cysteine endopeptidases Inonotus lanostane triterpenoids Basidiomycota Fungi Peptidases Inotodiol Cancerology Cell death Hydrolases Inhibitor Apoptosis caspase-3 |
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Snippet | Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids
from a chloroform extract... To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I.... BACKGROUNDTo investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of... |
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SubjectTerms | Animals Apoptosis - drug effects Apoptosis - physiology Basidiomycota - chemistry Biological and medical sciences Caspase 3 - metabolism Cell Growth Processes - drug effects Enzyme Activation - drug effects Female Lanosterol - analogs & derivatives Lanosterol - pharmacology Leukemia P388 - drug therapy Leukemia P388 - enzymology Leukemia P388 - pathology Medical sciences Mice Tumors |
Title | Inotodiol, a Lanostane Triterpenoid, from Inonotus obliquus Inhibits Cell Proliferation through Caspase-3-dependent Apoptosis |
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