Inotodiol, a Lanostane Triterpenoid, from Inonotus obliquus Inhibits Cell Proliferation through Caspase-3-dependent Apoptosis
Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. Materials and Methods: Cell viability was measured by MTT assay. Casp...
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Published in | Anticancer research Vol. 28; no. 5A; pp. 2691 - 2696 |
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Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
Attiki
International Institute of Anticancer Research
01.09.2008
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Subjects | |
Online Access | Get full text |
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Summary: | Background: To investigate the antitumor effect of Inonotus obliquus Pilat, the antiproliferative effect of lanostane triterpenoids
from a chloroform extract of I. obliquus sclerotia against mouse leukemia P388 cells was assessed. Materials and Methods:
Cell viability was measured by MTT assay. Caspase-3/7 activity and DNA fragmentation were evaluated to analyze apoptosis induction.
The in vivo antitumor effect was evaluated by the number of survival days of mouse leukemia P388-bearing female CDF 1 mice. Results: The chloroform extract of I. obliquus sclerotia inhibited proliferation of the P388 cells. Among the triterpenoids
examined, only inotodiol inhibited P388 cell proliferation. DNA fragmentation and caspase-3/7 activation were observed in
the P388 cells treated with inotodiol (30 μM). A caspase-3 inhibitor, DEVD-CHO (N-acetyl-Asp-Glu-Val-Asp-al, 100 μM) partially
inhibited the DNA fragmentation and growth-inhibition induced by inotodiol. The intraperitoneal administration of 10 mg/kg
inotodiol prolonged the number of survival days of the P388-bearing mice. Conclusion: Inotodiol inhibits cell proliferation
through apoptosis induction by activating caspase-3. |
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Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0250-7005 1791-7530 |