Decrease of Telomeres and Increase of Interstitial Telomeric Sites in Chromosomes of Short-term Cultured Gastric Carcinoma Cells Detected by Fluorescence In Situ Hybridization
Background: Deletion or shortening of chromosomal telomeres is associated with cellular aging and carcinogenesis. Telomeric sites are interstitially located in chromosomes. To clarify the frequency of telomerase abnormalities in cancer and their relationship with any characteristics of gastric carci...
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Published in | Anticancer research Vol. 26; no. 4B; pp. 2849 - 2855 |
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Main Authors | , , , , , , , , , |
Format | Journal Article |
Language | English |
Published |
Attiki
International Institute of Anticancer Research
01.07.2006
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Subjects | |
Online Access | Get full text |
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Summary: | Background: Deletion or shortening of chromosomal telomeres is associated with cellular aging and carcinogenesis. Telomeric
sites are interstitially located in chromosomes. To clarify the frequency of telomerase abnormalities in cancer and their
relationship with any characteristics of gastric carcinomas, telomeric aberrations in eleven cultured specimens of human gastric
cancer were investigated by cytogenetic analysis. Materials and Methods: Chromosomal metaphase specimens, obtained by primary
culture of cells from surgical specimens of eleven gastric cancer patients, were examined by fluorescent in situ hybridization
(FISH) using all telomeric and chromosome 17 specific-telomeric DNA probes. The number of telomeric signals and interstitial
telomeric signals (ITS) were counted. DNA ploidy was examined by flow cytometry. Results: The mean telomere signal per nucleus
(MTS) observed in peripheral blood lymphocytes (PBLs) of normal volunteers (n=10) was 71.3±3.9%. The MTS in the carcinoma
cells (46.9±2.6%) was significantly lower than in PBLs (p<0.01). Although ITS were not observed in PBLs, the mean rate of
ITS was 41.2±22.0%, and the mean rate of ITS per chromosome was 2.1±2.1% in the cancer specimens. In DNA aneuploid carcinoma
cells, the MTS was significantly lower, the mean rate of ITS tended to be higher (p=0.072), and the mean rate of ITS per chromosome
was significantly higher (p<0.05), than in DNA diploid lymphocytes. Histologically, the mean rate of ITS per chromosome in
carcinomas with venous infiltrations was significantly greater than in those without (p<0.05). Conclusion: Deletion and interstitial
translocation of telomeric loci of chromosomes were frequent alterations in gastric carcinoma cells and increased numbers
of interstitial telomeric signals were associated with venous invasion. |
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ISSN: | 0250-7005 1791-7530 |