Cytotoxicity of Clostridium difficile toxin A for human colonic and pancreatic carcinoma cell lines

The use of bacterial exotoxins may constitute novel adjuncts to treatment of gastrointestinal tract malignancies. Clostridium difficile toxin A was evaluated for its cytotoxic effect in vitro on 24 human cell lines and strains including carcinomas of the colon, pancreas, prostate, lung, breast, and...

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Bibliographic Details
Published inCancer research (Chicago, Ill.) Vol. 52; no. 18; pp. 5096 - 5099
Main Authors KUSHNARYOV, V. M, REDLICH, P. N, SEDMAK, J. J, LYERLY, D. M, WILKINS, T. D, GROSSBERG, S. E
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 15.09.1992
Subjects
Antineoplastic agents
Bacterial Toxins - toxicity
Biological and medical sciences
Carcinoma - drug therapy
Cell Survival - drug effects
Clostridium difficile
Colonic Neoplasms - drug therapy
Enterotoxins - toxicity
General aspects
Humans
In Vitro Techniques
Medical sciences
Pancreatic Neoplasms - drug therapy
Pharmacology. Drug treatments
Time Factors
Tumor Cells, Cultured - drug effects
Antineoplastic agent
Human
Carcinoma
Clostridium difficile
Clostridiales
Cytotoxicity
Malignant tumor
In vitro
Toxin
Cell line
Microbial origin
Clostridiaceae
Digestive diseases
Bacteria
Colon
Exotoxin
Pancreas
Tumor cell
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Summary:The use of bacterial exotoxins may constitute novel adjuncts to treatment of gastrointestinal tract malignancies. Clostridium difficile toxin A was evaluated for its cytotoxic effect in vitro on 24 human cell lines and strains including carcinomas of the colon, pancreas, prostate, lung, breast, and lymphoid malignancies, as well as nonmalignant tissues. All nine colon and five pancreas cell lines were extraordinarily sensitive to the cytotoxic effect of Clostridium difficile toxin A at very low concentrations. This effect, which occurred rapidly and was dose dependent, was observed in all cells of seven colon and two pancreas cell lines at concentrations as low as 1-5 ng/ml (10(-12) to 10(-11) M), whereas cells derived from other sites required 60 to greater than 500 ng/ml to achieve an equivalent effect. The data suggest that Clostridium difficile toxin A may have potential therapeutic value in the treatment of some gastrointestinal tract cancers.
ISSN:0008-5472
1538-7445