Renal Cell Carcinoma-induced Immunosuppression: An Immunophenotypic Study of Lymphocyte Subpopulations and Circulating Dendritic Cells

• Published in: Anticancer research Vol. 27; no. 1A; pp. 165 - 173
• Main Authors: PORTA, Camillo, BONOMI, Lucia, ROVERETO, Bruno, LILLAZ, Beatrice, PAGLINO, Chiara, ROVATI, Bianca, IMARISIO, Ilaria, MORBINI, Patrizia, VILLA, Chiara, DANOVA, Marco, MENSI, Mario
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.01.2007
• Subjects: Adult; Aged; Aged, 80 and over; Biological and medical sciences; Carcinoma, Renal Cell - blood; Carcinoma, Renal Cell - immunology; Carcinoma, Renal Cell - pathology; Dendritic Cells - immunology; Enzyme-Linked Immunosorbent Assay; Female; Humans; Immunohistochemistry; Immunophenotyping; Kidney Neoplasms - blood; Kidney Neoplasms - immunology; Kidney Neoplasms - pathology; Kidneys; Lymphocyte Subsets - immunology; Male; Medical sciences; Middle Aged; Neoplasm Staging; Nephrology. Urinary tract diseases; T-Lymphocytes - immunology; Tumors; Tumors of the urinary system; Kidney disease; Dendritic cell; Urinary system disease; Carcinoma; Immunophenotype; lymphocytic subpopulations; Malignant tumor; Cell subpopulation; Immunosuppression; Cancerology; dendritic cells; Antigen presenting cell; Renal cell carcinoma; Kidney cancer; Grawitz tumor; Lymphocyte
• ISSN: 0250-7005; 1791-7530

Background: Renal cell carcinoma (RCC)-induced immune dysfunction in patients at first diagnosis was investigated. Patients and Methods: The main circulating lymphocyte subsets, the total number of circulating and intratumor dendritic cells and the titers of circulating VEGF were quantified in 47 RCC patients, using flow cytometric, immunohistochemical and ELISA assays. Results: Despite a significant activation of CD3/HLA-DR + lymphocytes and of the CD56 + NK subset, RCC patients presented a marked immunosuppression of CD4/CD45RA naïve T-cells, CD4/CD45RO memory T-cells, CD16 + NK-cells, and total circulating dendritic cells, as well as a significant increase of lymphocytes co-expressing the CD4 and CD8 antigens. Finally, CD16 + /CD56 + NK and DCs were poorly represented in tumor specimens. Conclusion: The complex immunological dysfunctions demonstrated involve different levels of immunocompetence and indicate a pattern of major disturbance of the immune system.