The immune response to p53 in breast cancer patients is directed against immunodominant epitopes unrelated to the mutational hot spot

• Published in: Cancer research (Chicago, Ill.) Vol. 52; no. 22; pp. 6380 - 6384
• Main Authors: SCHLICHTHOLZ, B, LEGROS, Y, GLLET, D, GAILLARD, C, MARTY, M, LANE, D, CALVO, F, SOUSSI, T
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 15.11.1992
• Subjects: Antibodies, Neoplasm - blood; Antibodies, Neoplasm - genetics; Antibodies, Neoplasm - immunology; Biological and medical sciences; Breast Neoplasms - genetics; Breast Neoplasms - immunology; Female; Genes, p53 - genetics; Gynecology. Andrology. Obstetrics; Humans; Immunodominant Epitopes - analysis; Immunodominant Epitopes - immunology; Mammary gland diseases; Medical sciences; Mutation; Peptide Fragments - immunology; Prognosis; Tumor Suppressor Protein p53 - genetics; Tumor Suppressor Protein p53 - immunology; Tumors; Histological grading; Human; Prognosis; Immune response; Antibody; Gene product; Serology; Malignant tumor; Blood; Assay; Mammary gland diseases; Serum; Mammary gland; Mutation; Humoral immunity; Tumor suppressor gene
• ISSN: 0008-5472; 1538-7445

Alteration of the p53 gene is the most frequent genetic feature of human cancer and leads to overexpression of the altered protein in the tumor cell nucleus. Two diagnostic procedures are currently available to assess p53 mutations: (a) molecular analysis of the gene sequence; and (b) immunohistochemical analysis of p53 protein accumulation. We now report a third approach, serological analysis. Fifteen % of primary breast cancer patients were found to have circulating antibodies to p53 protein by immunoprecipitation or immunoblotting. We have found a close correlation between the presence of such antibodies and bad prognosis such as high histological grade and the absence of hormone receptors. Furthermore, we found that the B-cell response to p53 protein is induced by two immunodominant regions located at the carboxy and amino termini of the protein, outside the central mutational hot spot region. These findings suggest that serological analysis, combined with molecular and histochemical methods, may be suitable for assessing the state of the p53 gene in cancer patients.