Nuclear matrix of calreticulin in hepatocellular carcinoma

Nuclear matrix protein profiles of malignant cells vary from their normal counterparts. By two-dimensional gel electrophoresis, we analyzed nuclear matrix proteins in 11 hepatocellular carcinomas and compared them with corresponding non-neoplastic liver tissue. Although the compositions were mostly...

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Published inCancer research (Chicago, Ill.) Vol. 60; no. 4; pp. 1117 - 1120
Main Authors YOON, G.-S, LEER, H, JUNG, Y, YU, E, MOON, H.-B, SONG, K, LEE, I
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 15.02.2000
Subjects
Adult
Aged
Biological and medical sciences
Calcium-Binding Proteins - analysis
Calreticulin
Carcinoma, Hepatocellular - chemistry
Gastroenterology. Liver. Pancreas. Abdomen
Humans
Liver Neoplasms - chemistry
Liver. Biliary tract. Portal circulation. Exocrine pancreas
Medical sciences
Microscopy, Fluorescence
Middle Aged
Nuclear Matrix - chemistry
Ribonucleoproteins - analysis
Tumors
Human
Coiled structure
Tumoral tissue
Hepatic disease
Hepatocellular carcinoma
Identification
Malignant tumor
Nuclear envelope
Protein
Calreticulin
Digestive diseases
Localization
Tumor cell
Aminoacid sequence
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Summary:Nuclear matrix protein profiles of malignant cells vary from their normal counterparts. By two-dimensional gel electrophoresis, we analyzed nuclear matrix proteins in 11 hepatocellular carcinomas and compared them with corresponding non-neoplastic liver tissue. Although the compositions were mostly similar, several peptides were noted predominantly in the former. The most prominent one was an acidic protein of apparent Mr 62,000, which was identified to be calreticulin upon NH2-terminal amino acid sequencing. By immunoblotting, calreticulin was confirmed to be present abundantly in the nuclear matrix fraction of carcinomas but not in that of the nonmalignant liver tissue. Interestingly, the total content of calreticulin was similar between them. By immunofluorescence microscopy, evident nuclear immunostaining was detected in carcinomas. Calreticulin was also found to be in the nuclear matrices of various carcinoma cell lines. We conclude that calreticulin is a component of the nuclear matrix. The formation and/or expansion of the calreticulin-nuclear matrix may be related to the activated cell growth.
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ISSN:0008-5472
1538-7445