5-Fluorouracil-related Gene Expression in Hepatic Artery Infusion-treated Patients with Hepatic Metastases from Colorectal Carcinomas

• Published in: Anticancer research Vol. 28; no. 1B; pp. 389 - 393
• Main Authors: SAMESHIMA, Shinichi, TOMOZAWA, Shigeru, HORIKOSHI, Hiroyuki, MOTEGI, Kenta, HIRAYAMA, Isao, KOKETSU, Shinichiro, OKADA, Toshiyuki, KOJIMA, Masaru, KON, Youichi, SAWADA, Toshio
• Format: Journal Article
• Language: English
• Published: Attiki International Institute of Anticancer Research 01.01.2008
• Subjects: Aged; Antimetabolites, Antineoplastic - administration & dosage; Biological and medical sciences; Colorectal Neoplasms - drug therapy; Colorectal Neoplasms - enzymology; Colorectal Neoplasms - genetics; Colorectal Neoplasms - pathology; Dihydrouracil Dehydrogenase (NADP) - biosynthesis; Dihydrouracil Dehydrogenase (NADP) - genetics; Female; Fluorouracil - administration & dosage; Gastroenterology. Liver. Pancreas. Abdomen; Gene Expression - drug effects; Hepatic Artery; Humans; Infusions, Intra-Arterial; Liver Neoplasms - drug therapy; Liver Neoplasms - enzymology; Liver Neoplasms - genetics; Liver Neoplasms - secondary; Male; Medical sciences; Orotate Phosphoribosyltransferase - biosynthesis; Orotate Phosphoribosyltransferase - genetics; RNA, Messenger - biosynthesis; RNA, Messenger - genetics; Stomach. Duodenum. Small intestine. Colon. Rectum. Anus; Thymidine Phosphorylase - biosynthesis; Thymidine Phosphorylase - genetics; Thymidylate Synthase - biosynthesis; Thymidylate Synthase - genetics; Tumors; Antineoplastic agent; dihydropyrimidine dehydrogenase; Rectal disease; Angiogenic factor; Colorectal carcinoma; Liver; Glycosyltransferases; Colorectal cancer; Metastasis; Synthase; Cancerology; Intestinal disease; oroteta phophoribosyl transferase; Thymidine phosphorylase; Fluorouracil; Hepatic artery; Human; Digestive system; Enzyme; Fluoropyrimidine derivatives; Transferases; Enzyme inhibitor; ); Malignant tumor; Dihydropyrimidine dehydrogenase (NADP; Thymidylate synthase; Gene expression; Colonic disease; Treatment; Antimetabolic; Methyltransferases; Perfusion; Thymidine synthase; Pyrimidine derivatives; Digestive diseases; Oxidoreductases; Pentosyltransferases; Cancer
• ISSN: 0250-7005; 1791-7530

Aim: To predict the therapeutic efficacy of hepatic arterial infusion (HAI) with 5-fluorouracil (5FU) for patients with liver metastases from colorectal carcinomas, 5FU-related gene expressions were examined in primary colorectal carcinomas. Patients and Methods: Thirty-eight patients with liver metastases from colorectal carcinoma received HAI of 5FU. The expressions of the mRNAs for thymidine synthase (TS), dihydropyrimidine dehydrogenase (DPD), thymidine phosphorylase (TP), and oroteta phophoribosyl transferase (OPRT) in primary colorectal carcinomas were measured by RT-PCR. Results: The response rate was 52.6% (20/38). The overall median survival time was 29.1 months. DPD and TP expression was significantly higher in the progressive disease (PD) group than in the complete response (CR) or partial response (PR) group (p=0.032, p=0.014), respectively. The levels of DPD and TP mRNAs showed a significant correlation (r=0.76, p=0.0001). Conclusion: The expression of DPD and TP mRNAs in primary colorectal carcinomas was significantly predictive of the therapeutic response to 5FU HAI.