Enhanced Expression of Tie2, Its Ligand Angiopoietin-1, Vascular Endothelial Growth Factor, and CD31 in Human Non-Small Cell Lung Carcinomas

• Published in: Clinical cancer research Vol. 5; no. 9; pp. 2506 - 2510
• Main Authors: TAKAHAMA, M, TSUTSUMI, M, TSUJIUCHI, T, NEZU, K, KUSHIBE, K, TANIGUCHI, S, KOTAKE, Y, KONISHI, Y
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.09.1999
• Subjects: Adult; Aged; Aged, 80 and over; Angiopoietin-1; Biological and medical sciences; Biomarkers; Carcinoma, Non-Small-Cell Lung - blood supply; Carcinoma, Non-Small-Cell Lung - enzymology; Carcinoma, Non-Small-Cell Lung - metabolism; Endothelial Growth Factors - biosynthesis; Female; Humans; Ligands; Lung Neoplasms - blood supply; Lung Neoplasms - enzymology; Lung Neoplasms - metabolism; Lymphokines - biosynthesis; Male; Medical sciences; Membrane Glycoproteins - biosynthesis; Middle Aged; Neovascularization, Pathologic - genetics; Neovascularization, Pathologic - metabolism; Platelet Endothelial Cell Adhesion Molecule-1 - biosynthesis; Pneumology; Receptor Protein-Tyrosine Kinases - biosynthesis; Receptor, TIE-2; Ribonucleases - metabolism; Tumors of the respiratory system and mediastinum; Vascular Endothelial Growth Factor A; Vascular Endothelial Growth Factors; Human; Lung disease; Angiogenic factor; Respiratory disease; Tumoral marker; Malignant tumor; Gene expression; Bronchopulmonary; Angiogenesis; Vascular endothelium growth factor; Messenger RNA; Bronchus disease; Non small cell carcinoma
• ISSN: 1078-0432; 1557-3265

Expression of angiogenesis-associated genes was compared in 32 primary non-small cell lung carcinoma samples (14 adenocarcinomas, 17 squamous cell carcinomas, and 1 large cell carcinoma) and paired adjacent noncancerous lung tissues using a multiprobe RNase protection assay. Levels of Tie2, angiopoietin (Ang)-1, vascular endothelial growth factor (VEGF), and CD31 mRNAs were higher in cancers than in adjacent noncancerous tissues, in contrast to the fms-like tyrosine kinase (Flt)-1, Flt-4, Tie1, thrombin receptor, endoglin, and VEGF-C, for which no differences were evident. Overexpression did not seem to differ with histological type and pathological stage. Significant positive correlations were found between mRNA expression of Ang-1 and those of Tie2 and CD31, and that of VEGF and those of Flt-1 and CD31. These findings suggest that Ang-1 and VEGF are important angiogenic factors in human non-small cell lung carcinomas.