Carcinogenesis in heterotopic respiratory epithelium in canine subcutaneous bronchial autografts

Short bronchial segments obtained by pneumonectomy were implanted, 9-12 per dog, in the subcutaneous tissues of the back of seven dogs. These subcutaneous bronchial autografts (SBA) became vascularized, and they contained viable, histologically normal respiratory epithelium 4 wk after implantation....

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Published inCancer research (Chicago, Ill.) Vol. 46; no. 6; pp. 2995 - 2999
Main Authors HAMMOND, W. G, BENFIELD, J. R, PALADUGU, R. R, AZUMI, N, PAK, H. Y, TEPLITZ, R. L
Format Journal Article
LanguageEnglish
Published Philadelphia, PA American Association for Cancer Research 01.06.1986
Subjects
Animal tumors. Experimental tumors
Animals
Biological and medical sciences
Bronchi - pathology
Bronchi - transplantation
Bronchial Neoplasms - etiology
Bronchial Neoplasms - pathology
Disease Models, Animal
DNA - analysis
Dogs
Epithelium - pathology
Experimental respiratory system tumors
Female
Male
Medical sciences
Methylcholanthrene
Precancerous Conditions - pathology
Transplantation, Autologous
Tumors
Fissipedia
Carnivora
Vertebrata
Autograft
Mammalia
Respiratory disease
Subcutaneous
Experimental tumor
Carcinogenesis
Dog
Bronchopulmonary
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Summary:Short bronchial segments obtained by pneumonectomy were implanted, 9-12 per dog, in the subcutaneous tissues of the back of seven dogs. These subcutaneous bronchial autografts (SBA) became vascularized, and they contained viable, histologically normal respiratory epithelium 4 wk after implantation. From 1-3 mo after implantation, 10% methylcholanthrene in steroid suspension medium was instilled into 21 SBAs, and 10% methylcholanthrene in a silicone polymer sustained release implant was placed in 22 SBAs. Ten SBAs were left carcinogen free as controls. SBA contents were examined cytologically at 3-mo intervals. Biopsies were done from 2-32 mo after carcinogen implantation. Progressive preneoplastic changes were noted in all five dogs which received carcinogen. Curetments of five SBAs after 14-mo exposure to methylcholanthrene yielded 10(4)-10(5) cells from each SBA; 40-70% of the cells obtained were at the same stage of atypical squamous metaplasia. At least one SBA in each dog yielded cancer cells by cytological criteria by 19-29 mo after instillation. Biopsy of a grossly abnormal SBA revealed well-differentiated epidermoid carcinoma at 32 mo. The multiple SBA method provides isolated portions of canine respiratory epithelium for the study of chemical carcinogenesis and for the production of sizable preneoplastic cell populations.
ISSN:0008-5472
1538-7445