Ultraviolet-induced cyclobutane pyrimidine dimers are selectively removed from transcriptionally active genes in the epidermis of the hairless mouse

• Published in: Cancer research (Chicago, Ill.) Vol. 53; no. 7; pp. 1642 - 1645
• Main Authors: RUVEN, H. J. T, BERG, R. J. W, SEELEN, C. M. J, DEKKERS, J. A. J. M, LOHMAN, P. H. M, MULLENDERS, L. H. F, VAN ZEELAND, A. A
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 01.04.1993
• Subjects: Adenosine Deaminase - genetics; Animals; Biological and medical sciences; Carcinogenesis, carcinogens and anticarcinogens; DNA - isolation & purification; DNA - radiation effects; DNA Repair; Genes, mos; Haptoglobins - genetics; Hypoxanthine Phosphoribosyltransferase - genetics; Male; Medical sciences; Mice; Mice, Hairless; Physical agents; Pyrimidine Dimers - metabolism; Skin - radiation effects; Tumors; Ultraviolet Rays; Transcription; UVB radiation; Toxicity; Rodentia; Single dose; Epidermis; Carcinogen; Vertebrata; Specificity; Mammalia; Gene; Ultraviolet irradiation; Mouse; Animal; DNA; Dimer; Skin; Repair; Pyrimidine nucleotide; Molecular adduct
• ISSN: 0008-5472; 1538-7445

This study describes the induction and repair of UV-induced cyclobutane pyrimidine dimers (CPD) in transcriptionally active and inactive genes in the epidermis of the hairless mouse. Mice were exposed to a single dose of 2000 J/m2 ultraviolet B and kept in darkness for up to 24 h. The CPD frequency was measured in the transcriptionally active hypoxanthine-guanine phosphoribosyltransferase gene, the adenosine deaminase gene, the inactive c-mos protooncogene, and the haptoglobin gene using the CPD-specific enzyme T4 endonuclease V. Sixty % of the CPD was removed from the active genes during the first 4 h, after which no further repair took place up to 24 h. In contrast, the inactive genes did not show any removal of CPD. Assuming that the rate of repair in the c-mos and haptoglobin genes is representative for the repair rate in the genome overall, these results suggest only marginal repair of UV-induced CPD in the mouse epidermis in vivo. The selective repair of active genes in the epidermis of mice resembles that of rodent cells in culture and shows the biological relevance of repair studies performed with cultured rodent cells in vitro.