Mitochondrial dysfunction after aerobic exposure to the hypoxic cytotoxin tirapazamine

• Published in: Cancer research (Chicago, Ill.) Vol. 61; no. 1; pp. 145 - 152
• Main Authors: WOUTERS, Bradly G, DELAHOUSSAYE, Yvette M, EVANS, James W, BIRRELL, Geoffrey W, DORIE, Mary Jo, JINGLI WANG, MACDERMED, Dhara, CHIU, Roland K, BROWN, J. Martin
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 2001
• Subjects: Aerobiosis; Animals; Antineoplastic Agents - metabolism; Antineoplastic Agents - toxicity; Biological and medical sciences; Cell Membrane Permeability - drug effects; Cell Membrane Permeability - physiology; CHO Cells - drug effects; Cricetinae; Crosses, Genetic; Drug toxicity and drugs side effects treatment; Electron Transport - physiology; Humans; Intracellular Membranes - drug effects; Intracellular Membranes - physiology; Medical sciences; Membrane Potentials - drug effects; Mice; Mice, Inbred BALB C; Mice, Knockout; Miscellaneous (drug allergy, mutagens, teratogens...); Mitochondria - drug effects; Mitochondria - metabolism; Mitochondria - physiology; Mitochondria, Muscle - drug effects; Mitochondria, Muscle - metabolism; Mitochondria, Muscle - physiology; Oxygen - metabolism; Pharmacology. Drug treatments; Superoxide Dismutase - genetics; Superoxide Dismutase - metabolism; Superoxides - metabolism; Triazines - metabolism; Triazines - toxicity; Tumor Cells, Cultured; Antineoplastic agent; Toxicity; Superoxide dismutase; Dose activity relation; Mitochondria; Bioreductive drug; Established cell line; Muscle; Membrane potential; Mechanism of action; Tirapazamine; Cell permeability; Enzyme; Cytochrome P450; Rodentia; Aerobe; Metabolism; In vitro; Transitory; In vivo; Vertebrata; Mammalia; Mouse; Animal; Oxidoreductases; Intracellular; Reductase
• ISSN: 0008-5472; 1538-7445

Tirapazamine (TPZ) is a bioreductive drug that exhibits a high degree of selective toxicity toward hypoxic cells, and at doses that are used clinically, little or no cell killing is observed in aerobic cells. Nonetheless, the effects of TPZ on aerobic tissues are still responsible for the dose limitations on the clinical administration of this drug. Clinical side effects include fatigue, muscle cramping, and reversible ototoxicity. We have investigated TPZ-induced changes in the mitochondria in aerobically exposed cells as a potential mediator of these side effects. Our data show that aerobic administration of TPZ at clinically relevant doses results in a profound loss in the mitochondrial membrane potential (MMP). We show that loss in the MMP occurs in a variety of cell lines in vitro and also occurs in muscle tissues in vivo. The loss in MMP is temporary because recovery occurs within 2 h. TPZ is directly metabolized within mitochondria to a DNA-damaging form, and this metabolism leads to both the cell-killing effects of TPZ on aerobic cells at high doses and to the loss in MMP at clinically relevant doses. Using cell lines derived from genetically modified mice with a targeted deletion in manganese superoxide dismutase, we have further distinguished the phenotypic effects of TPZ in mitochondria at high toxic doses versus those at clinically relevant doses. We have investigated several potential mechanisms for this TPZ-induced loss in MMP. Our results indicate no change in the rate of cellular respiration in TPZ-treated cells. This implies that the loss in MMP results from an inability of the inner mitochondrial membrane to sustain a potential across the membrane after TPZ treatment. Incubation of cells with an inhibitor of the mitochondrial permeability transition suggests that the loss of MMP may result from the regulated opening of a large mitochondria channel.