Somatostatin Inhibits Follicle-Stimulating Hormone-Induced Adenylyl Cyclase Activity and Proliferation in Immature Porcine Sertoli Cell via sst2 Receptor

• Published in: Biology of reproduction Vol. 62; no. 6; pp. 1835 - 1843
• Main Authors: KRANTIC, S, BENAHMED, M
• Format: Journal Article
• Language: English
• Published: Madison, WI Society for the Study of Reproduction 01.06.2000
• Subjects: Adenylate Cyclase Toxin; Adenylyl Cyclase Inhibitors; Adenylyl Cyclases - metabolism; Animals; Biological and medical sciences; Cell Division - drug effects; Colforsin - pharmacology; Cyclic AMP - biosynthesis; DNA - biosynthesis; Enzyme Inhibitors - pharmacology; Follicle Stimulating Hormone - pharmacology; Fundamental and applied biological sciences. Psychology; Male; Mammalian male genital system; Morphology. Physiology; Pertussis Toxin; Receptors, Somatostatin - physiology; Sertoli Cells - cytology; Sertoli Cells - drug effects; Sertoli Cells - enzymology; Somatostatin - pharmacology; Swine; Vertebrates: reproduction; Virulence Factors, Bordetella - pharmacology; Cell proliferation; Sertoli cell; Enzyme; Phosphorus-oxygen lyases; Lyases; Testicle; Male genital system; Pig; Adenylate cyclase; Vertebrata; Mammalia; Enzymatic activity; Artiodactyla; Somatostatin; Immaturity; Ungulata; Hormonal receptor
• ISSN: 0006-3363; 1529-7268
• DOI: 10.1095/biolreprod62.6.1835

The potential involvement of somatostatin (SRIF) in testicular function was studied by using as a model primary cultures of purified immature porcine Sertoli cells. In the present report we show that Sertoli cells express mRNA for sst2 SRIF receptor and display SRIF-sensitive adenylyl cyclase. Sensitivity of adenylyl cyclase to SRIF and its analogues is compatible with the pharmacological profile of this receptor type. Relevant cAMP production is similarly inhibited by SRIF in both basal and stimulated (by gonadotropin FSH or by forskolin) conditions. Moreover, the observed SRIF actions on Sertoli cells require functional coupling of specific membrane receptors to adenylyl cyclase via Gi proteins because pertussis toxin prevents SRIF-dependent inhibition of adenylyl cyclase in either basal or FSH-stimulated conditions. Given the potent antiproliferative actions of SRIF in other cell types, we further assessed the possible SRIF-dependent modulation of [ 3 H]thymidine incorporation by Sertoli cells. Our data point to SRIF-mediated inhibition of both basal and FSH-stimulated [ 3 H]thymidine uptake. This inhibition of Sertoli cell proliferation is, at least in basal conditions, also blocked by pertussis toxin pretreatment. Altogether, these data suggest that SRIF may play a role as an (local) inhibitor of FSH actions in testicular development.