Numerical Aberrations of Chromosome 8 in Gastric Cancer Detected by Fluorescence In Situ Hybridization

Background: Limited data are available on the genetic events underlying gastric cancer. Studying a few cases by conventional cytogenetic techniques, we previously reported that chromosome 8 might be frequently involved. The aim of our study was to evaluate the numerical aberrations of chromosome 8 i...

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Published inAnticancer research Vol. 24; no. 1; pp. 155 - 159
Main Authors PANANI, Anna D, FERTI, Angeliki D, AVGERINOS, Alexandros, RAPTIS, Satirios A
Format Journal Article
LanguageEnglish
Published Attiki International Institute of Anticancer Research 01.01.2004
Subjects
Aneuploidy
Biological and medical sciences
Chromosome Aberrations
Chromosomes, Human, Pair 8 - genetics
Gastroenterology. Liver. Pancreas. Abdomen
Humans
In Situ Hybridization, Fluorescence
Medical sciences
Stomach Neoplasms - genetics
Stomach Neoplasms - pathology
Stomach. Duodenum. Small intestine. Colon. Rectum. Anus
Tumors
Interphase
Chromosome 8
Fluorescence in situ hybridization
Cytogenetics
Digestive diseases
Malignant tumor
Stomach cancer
Gastric disease
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Summary:Background: Limited data are available on the genetic events underlying gastric cancer. Studying a few cases by conventional cytogenetic techniques, we previously reported that chromosome 8 might be frequently involved. The aim of our study was to evaluate the numerical aberrations of chromosome 8 in gastric cancer using fluorescence in situ hybridization (FISH). Materials and Methods: FISH, with an a-satellite DNA probe specific for chromosome 8, was applied to 37 primary gastric tumors directly processed for cytogenetic study. Results: Numerical aberrations of chromosome 8 were observed in 23 out of 37 tumors (62.16%). Trisomy was detected in 16 cases (43.24%), tetrasomy in 4 cases (10.81%) and monosomy in 3 cases (8.10%). No correlation was found between polysomy 8 and the histopathologic characteristics of the tumors. Conclusion: An increase in the number of chromosome 8 may frequently occur in gastric cancer. Advanced and more aggressive gastric tumors did not harbor polysomy 8. Further studies at molecular and clinical level must be carried out to identify the gene alterations reflected by polysomy 8 and possibly to facilitate the detection of specific tumors subtypes.
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ISSN:0250-7005
1791-7530