A quantitative assay of retrograde transported HSV in the trigeminal ganglion

• Published in: Investigative ophthalmology & visual science Vol. 33; no. 6; pp. 1934 - 1939
• Main Authors: Carter, SR, Pereira, L, Paz, P, LaVail, JH
• Format: Journal Article
• Language: English
• Published: Rockville, MD ARVO 01.05.1992; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Biological and medical sciences; Cornea - innervation; Cornea - microbiology; Cranial Nerve Diseases - microbiology; Disease Models, Animal; Experimental viral diseases and models; Female; Fluorescent Antibody Technique; Herpes Simplex - microbiology; Infectious diseases; Keratitis, Herpetic - microbiology; Male; Medical sciences; Mice; Mice, Inbred BALB C; Simplexvirus - physiology; Trigeminal Ganglion - microbiology; Viral diseases; Virus Replication; Infection; Virus; Cornea; Eye disease; Experimental disease; Herpesviridae; Animal; Retrograde; Viral disease; Alphaherpesvirinae; Exploration; Herpesvirus hominis
• ISSN: 0146-0404; 1552-5783

The relationship between the dose of Herpes simplex virus type 1 (HSV) inoculated in the cornea and the amount of actively replicating virus recovered from mouse trigeminal ganglion cells 5 d after corneal scratch and inoculation was investigated with a tissue culture plaque assay. A dose response curve of productive viral replication was obtained. The estimated dose of HSV that produces half-maximal recovery of virus within the ganglion was 9.15 x 10(3) plaque forming units per eye, and the maximal amount of HSV recovered was 1.34 x 10(4) pfu per ganglion. This definition of infectivity as a function of dose will be useful for studying the effects of potential inhibitors of the binding, uptake, and transport of HSV by productively or latently infected trigeminal neurons.