Antibody responses and infertility in mice following oral immunization with attenuated Salmonella typhimurium expressing recombinant murine ZP3
Ovarian ZP3, the primary sperm receptor, is a major glycoprotein of mouse zona pellucida (ZP). Because antibodies raised against ZP3 block sperm-egg interaction, ZP3 has been considered a candidate immunogen in the development of a contraceptive vaccine. This study explored the possibility of using...
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Published in | Biology of reproduction Vol. 56; no. 1; pp. 33 - 41 |
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Main Authors | , , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Society for the Study of Reproduction
01.01.1997
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Subjects | |
Online Access | Get full text |
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Summary: | Ovarian ZP3, the primary sperm receptor, is a major glycoprotein of mouse zona pellucida (ZP). Because antibodies raised against
ZP3 block sperm-egg interaction, ZP3 has been considered a candidate immunogen in the development of a contraceptive vaccine.
This study explored the possibility of using an attenuated Salmonella typhimurium vaccine strain expressing recombinant ZP3
to elicit an antibody response and infertility in mice. A cDNA sequence generated by the polymerase chain reaction encoding
342 amino acid residues (23-364) of the mouse (m)ZP3 was cloned into an Asd+ vector. An avirulent Salmonella vaccine strain
stably expressed the ZP3 polypeptide and colonized the internal organs of mice after oral inoculation. Oral immunization of
female BALB/c mice with the recombinant Salmonella vaccine strain expressing mZP3 induced significant levels of anti-native
ZP IgG antibodies in serum and IgA antibodies in vaginal secretions. The IgG antibodies thus induced also bound to ZP in vivo.
When mated with males, 3 of 6 females immunized with the recombinant Salmonella were infertile. In contrast, none of the mice
that received Salmonella containing the vector plasmid produced antibodies to ZP and all were fertile. No ovarian inflammation
was observed in the immunized mice at autopsy. The results suggest a potential oral contraceptive vaccine to control populations
of rodent vectors of disease and to induce reversible infertility in humans. |
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Bibliography: | ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-1 content type line 23 ObjectType-Article-1 ObjectType-Feature-2 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod56.1.33 |