Hyperthermic treatment of rabbit corneas

Well defined heat doses (temperature X time) were applied to normal rabbit corneas in an effort to determine thermal tolerance, and to examine the effects of heat on this tissue. A purely conductive heater was chosen to minimize intraocular penetration, and avoid findings attributable to nonthermal...

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Published inInvestigative ophthalmology & visual science Vol. 30; no. 8; pp. 1778 - 1783
Main Authors Goldblatt, WS, Finger, PT, Perry, HD, Stroh, EM, Weiser, DS, Donnenfeld, ED
Format Journal Article
LanguageEnglish
Published Rockville, MD ARVO 01.08.1989
Association for Research in Vision and Ophtalmology
Subjects
Animals
Biological and medical sciences
Cornea - pathology
Hot Temperature - adverse effects
Hyperthermia, Induced
Medical sciences
Ophthalmology
Rabbits
Temperature
Time Factors
Pathology
Vertebrata
Cornea
Eye disease
Mammalia
Rabbit
Lagomorpha
Hyperthermia
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Summary:Well defined heat doses (temperature X time) were applied to normal rabbit corneas in an effort to determine thermal tolerance, and to examine the effects of heat on this tissue. A purely conductive heater was chosen to minimize intraocular penetration, and avoid findings attributable to nonthermal effects of inductive sources. The etched element heater was sewn to 38 rabbit corneas. Thirty-six were treated to temperatures of 38, 45, 52 and/or 59 degrees centigrade for durations of 5, 15, or 45 min. Three eyes were treated at each time-temperature interval and sacrificed at either time 0, 1 day or 1 week follow-up. Histologic examinations were performed on all corneas. A corneal haze was first noted at 45 degrees C X 45 minutes X 1 day follow-up. This correlated with a mild stromal edema on light microscopy. Higher thermal doses produced a spectrum of damage, with complete destruction of all keratocytes and endothelial cells at 59 degrees C X 45 min. At levels greater than 45 degrees C x 45 min, heat damage was noted to be increased at 24 hr followup. Some recovery was noted by 1 week follow-up, with the exception of the 59 degrees C X 15 or 45 min groups. These two heat doses induced a drop-out of cellular elements with evidence of disintegration and fragmentation of collagen fibrils. Conductive heating of up to 45 degrees C X 15 min appeared well tolerated by normal rabbit corneas.
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ISSN:0146-0404
1552-5783