Retinal S-antigen and retinoblastoma: a monoclonal antibody and flow cytometric study

• Published in: Investigative ophthalmology & visual science Vol. 26; no. 4; pp. 568 - 571
• Main Authors: Donoso, LA, Felberg, NT, Augsburger, JJ, Shields, JA
• Format: Journal Article
• Language: English
• Published: Bethesda, MD ARVO 01.04.1985; Association for Research in Vision and Ophtalmology
• Subjects: Animals; Antibodies, Monoclonal - immunology; Antigens - analysis; Arrestin; Biological and medical sciences; Cell Cycle; Cell Line; Eye Neoplasms - immunology; Flow Cytometry; Medical sciences; Mice; Ophthalmology; Retina - immunology; Retinoblastoma - immunology; Tumors and pseudotumors of the eye, orbit, eyelid, lacrimal apparatus; Human; Immunohistochemistry; Flow cytometry; Eye disease; Immunological investigation; Infant; Retina; Tumor; Monoclonal antibody; Retinoblastoma; Immunological method
• ISSN: 0146-0404; 1552-5783

Retinal S-antigen was demonstrated in the WERI-Rb1 and to a lesser extent the Y-79 tissue cultured retinoblastoma cell lines as well as an ethanol-fixed, paraffin-embedded retinoblastoma by an indirect immunoperoxidase technique using monoclonal antibody MAbA9-C6, and by flow cytometric analysis (FCM) using MAbA9-C6 and MAbA1-G5. In fixed tissue sections, S-antigen immunoreactivity was restricted and localized to small numbers of retinoblastoma cells, including Flexner-Wintersteiner rosettes. By FCM, MAbA9-C6 bound to 15.4% of WERI-Rb1 cells and to 10.99.7% of Y-79 cells whereas MAbA1-G5 bound to 14.66% of WERI-Rb1 and to 4.23% of Y-79 cells respectively. Cell cycle analysis showed that S-antigen was predominately expressed in the resting (G0/G1) phase. The usefulness of MAbA9-C6 in studying the embryological development of the retina and as a marker protein for studying antigenic expression and modulation in retinoblastomas is discussed.