Isolation and identification of 3-carbamoyloxy-2-phenylpropionic acid as a major human urinary metabolite of felbamate

A new metabolite of felbamate, isolated from the polar metabolite fraction of a human urine sample, was identified as 3-carbamoyloxy-2-phenylpropionic acid (CPPA) by electron impact and CI/MS of the methyl ester in the isolated fraction. Its presence in the unconjugated free form in two different hu...

Full description

Saved in:
Bibliographic Details
Published inDrug metabolism and disposition Vol. 21; no. 4; p. 710
Main Authors Adusumalli, V E, Choi, Y M, Romanyshyn, L A, Sparadoski, Jr, R E, Wichmann, J K, Wong, K K, Kucharczyk, N, Sofia, R D
Format Journal Article
LanguageEnglish
Published United States 01.07.1993
Subjects
Animals
Anticonvulsants - isolation & purification
Anticonvulsants - metabolism
Anticonvulsants - pharmacology
Anticonvulsants - urine
Chromatography, High Pressure Liquid
Dogs
Humans
Male
Mass Spectrometry
Phenylcarbamates
Phenylpropionates - isolation & purification
Phenylpropionates - pharmacology
Phenylpropionates - urine
Propylene Glycols - metabolism
Rats
Rats, Sprague-Dawley
Online AccessGet more information

Cover

More Information
Summary:A new metabolite of felbamate, isolated from the polar metabolite fraction of a human urine sample, was identified as 3-carbamoyloxy-2-phenylpropionic acid (CPPA) by electron impact and CI/MS of the methyl ester in the isolated fraction. Its presence in the unconjugated free form in two different human urine samples was confirmed by HPLC comparison of retention times with authentic synthetic CPPA and by on-line negative ion HPLC thermospray tandem mass spectrometric techniques. The amount of CPPA in 0-4 hr postdose human urine was estimated to be approximately 12% of the drug-derived substances present in the urine. Some CPPA was also found to be present in dog urine.
ISSN:0090-9556