Douglas-fir : A third wood-drying condensate found to exhibit in vitro cytotoxicity and genotoxicity

The present study applies the approach described in Mark et al. for the testing of toxic chemicals produced during the drying of Douglas-fir. The genotoxic potential of Douglas-fir condensate has been previously unexplored and is thus an area of appropriate concern to the forest products industry, r...

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Bibliographic Details
Published inAnnals of clinical and laboratory science Vol. 26; no. 3; pp. 269 - 274
Main Authors MARK, H. F. L, NARAM, R, SINGER, J, RICE, R, LAMARCHE, P. H
Format Journal Article
LanguageEnglish
Published Philadelphia, PA Institute for Clinical Science 01.05.1996
Subjects
Animals
Biological and medical sciences
Cell Division
Cell Survival
CHO Cells
Chromosome Aberrations
Cricetinae
Medical sciences
Metaphase
Mutagenicity Tests
Mutagens - toxicity
Pesticides, fertilizers and other agrochemicals toxicology
Sister Chromatid Exchange
Toxicology
Trees - chemistry
Animal model
Wood
Rodentia
Chromosome
Cytotoxicity
In vitro
Vertebrata
Mammalia
Animal
Risk factor
Cytogenetics
Hamster
Drying
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Summary:The present study applies the approach described in Mark et al. for the testing of toxic chemicals produced during the drying of Douglas-fir. The genotoxic potential of Douglas-fir condensate has been previously unexplored and is thus an area of appropriate concern to the forest products industry, regulatory, agencies, and the general public. Previous research conducted in this laboratory has identified two wood-drying condensates that yield positive cytotoxic and genotoxic effects. The results of testing Southern yellow pine and Eastern white pine condensates have been reported elsewhere. Douglas-fir condensate, a third wood-drying condensate, was added in vitro in concentrations ranging from 0.1 to 100 microliters/ml to cultures of Chinese Hamster Ovary (CHO-WBL) cells. A dose response curve was observed with this condensate for both cytotoxicity and genotoxicity. The number of viable cells as well as the mitotic index (MI) and proliferative rate index (PRI) varied inversely with dosage. The result of chromosome aberration (Abs) analysis and sister chromatid exchange (SCE) analysis, both cytogenetic measures of genotoxicity, also gave statistically significant results.
ISSN:0091-7370
1550-8080