Requirement of a Centrosomal Activity for Cell Cycle Progression through G1 into S Phase

• Published in: Science (American Association for the Advancement of Science) Vol. 291; no. 5508; pp. 1547 - 1550
• Main Authors: Hinchcliffe, Edward H., Miller, Frederick J., Cham, Matthew, Khodjakov, Alexey, Sluder, Greenfield
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for the Advancement of Science 23.02.2001; American Association for the Advancement of Science; The American Association for the Advancement of Science
• Subjects: Animals; Antigens - metabolism; Biological and medical sciences; Cell cycle; Cell cycle, cell proliferation; Cell Division - drug effects; Cell Line; Cell nucleus; Cell physiology; Cells; Cellular biology; Centrioles; Centrioles - physiology; Centrosome - physiology; Centrosomes; Cercopithecus aethiops; Cytoplasmic Granules - physiology; Cytoplasmic Granules - ultrastructure; Daughter cells; Duplication; Fundamental and applied biological sciences. Psychology; G1 Phase; Interphase; Kidney cells; Microscopy, Video; Microtubule-Organizing Center - physiology; Microtubules - physiology; Microtubules - ultrastructure; Mitosis; Molecular and cellular biology; Monkeys & apes; Paclitaxel - pharmacology; S Phase; Somatic cells; Spindle Apparatus - physiology; Spindle Apparatus - ultrastructure; Tubulin - metabolism; Monkey; Vertebrata; G1 Phase; Mammalia; Tubulin; S Phase; Cell cycle; Primates; G2 Phase; Transition; Centriole; Centrosome; Microtubule organizing center; Karyoplaste
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.1056866

Centrosomes were microsurgically removed from BSC-1 African green monkey kidney cells before the completion of S phase. Karyoplasts (acentrosomal cells) entered and completed mitosis. However, postmitotic karyoplasts arrested before S phase, whereas adjacent control cells divided repeatedly. Postmitotic karyoplasts assembled a microtubule-organizing center containing γ-tubulin and pericentrin, but did not regenerate centrioles. These observations reveal the existence of an activity associated with core centrosomal structures-distinct from elements of the microtubule-organizing center-that is required for the somatic cell cycle to progress throughG1 into S phase. Once the cell is in S phase, these core structures are not needed for the G2-M phase transition.