TH1 and TH2 cell antigen receptors in experimental leishmaniasis

• Published in: Science (American Association for the Advancement of Science) Vol. 259; no. 5100; pp. 1457 - 1460
• Main Authors: REINER, S. L, ZHI-EN WANG, FARAH HATAM, SCOTT, P, LOCKSLEY, R. M
• Format: Journal Article
• Language: English
• Published: Washington, DC American Association for the Advancement of Science 05.03.1993
• Subjects: Animals; Base Sequence; Biological and medical sciences; CD4 Antigens - analysis; Cloning, Molecular; Human protozoal diseases; Infectious diseases; Leishmania tropica; Leishmaniasis, Cutaneous - immunology; Leshmaniasis; Medical sciences; Mice; Mice, Inbred BALB C; Molecular Sequence Data; Oligodeoxyribonucleotides; Parasitic diseases; Polymerase Chain Reaction - methods; Protozoal diseases; Receptors, Antigen, T-Cell - analysis; Receptors, Antigen, T-Cell - genetics; Receptors, Antigen, T-Cell - metabolism; Receptors, Antigen, T-Cell, alpha-beta - analysis; Receptors, Antigen, T-Cell, alpha-beta - genetics; Receptors, Antigen, T-Cell, alpha-beta - metabolism; Reference Values; RNA, Messenger - genetics; RNA, Messenger - isolation & purification; T-Lymphocyte Subsets - immunology; Tropical medicine; Protozoa; Protozoal disease; T cell receptor; Rodentia; Leishmaniasis; Infection; Antigen; Vertebrata; Experimental disease; Mammalia; Mouse; Animal; Rhizoflagellata; Lymphocyte; Leishmania major
• ISSN: 0036-8075; 1095-9203
• DOI: 10.1126/science.8451641

The complexity and chronicity of parasitic infections have obscured the identification of biologically relevant antigens. Analysis of the T cell receptor repertoire used by mice infected with Leishmania major revealed the expansion of a restricted population of CD4+ cells. These cells expressed the V alpha 8-J alpha TA72, V beta 4 heterodimer in both progressive infection and protective immunity and across several major histocompatibility haplotypes. Thus, the same immunodominant parasite epitope drives the disparate outcomes of this infectious process, suggesting that candidate vaccine antigens selected by screening of immune individuals may be capable of exacerbating disease in genetically susceptible individuals.