High tolerance against Chrysomela tremulae of transgenic poplar plants expressing a synthetic cry3Aa gene from Bacillus thuringiensis ssp tenebrionis

Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in f...

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Published inMolecular breeding Vol. 11; no. 2; pp. 103 - 110
Main Authors Génissel, Anne, Jean-Charles Leplé, Millet, Nadège, Augustin, Sylvie, Jouanin, Lise, Pilate, Gilles
Format Journal Article
LanguageEnglish
Published Dordrecht Springer Nature B.V 01.02.2003
Springer Verlag
Subjects
Biochemistry, Molecular Biology
Developmental stages
Gene expression
Genetic engineering
Life Sciences
Molecular biology
Plant biology
Poplar
Toxicity
Transgenic plants
PEUPLIER
TOXICITE DES FEUILLES
RESISTANCE AUX INSECTES NUISIBLES
CHRYSOMELA TREMULAE
BACILLUS THURINGIENSIS
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Summary:Hybrid poplars (Populus tremula ×Populus tremuloides) have been genetically engineered viaAgrobacterium tumefaciens, to express a syntheticcry3Aa gene derived from the native Bacillusthuringiensis subsp. tenebrionis cry3Aa gene.The presence and the expression of the transgene have been verified in fourtransgenic poplar lines, using Southern, northern and western analyses. Thetransgenic poplar's toxicity towards the phytophagous beetleChrysomela tremulae (Coleoptera, Chrysomelidae) has beenassessed on six month-old greenhouse-grown selected plants in laboratoryconditions. Laboratory experiments consisted of feeding tests of fresh detachedleaves on C. tremulae at all developmental stages. Ourresults indicate that the transgenic poplar leaves, expressing a Cry3Aa proteinamount in a range of 0.05–0.0025% of total soluble protein, weredefinitely deleterious for C. tremulae, regardless of thedevelopmental stage.
Bibliography:ObjectType-Article-2
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content type line 23
ISSN:1380-3743
1572-9788
DOI:10.1023/A:1022453220496