Detection of soluble angiotensin-converting enzyme 2 in heart failure: insights into the endogenous counter-regulatory pathway of the renin-angiotensin-aldosterone system

• Published in: Journal of the American College of Cardiology Vol. 52; no. 9; pp. 750 - 754
• Main Authors: Epelman, Slava, Tang, W H Wilson, Chen, Stephen Y, Van Lente, Frederick, Francis, Gary S, Sen, Subha
• Format: Journal Article
• Language: English
• Published: United States Elsevier Limited 26.08.2008
• Subjects: Acute coronary syndromes; Aged; Cardiology; Cardiovascular disease; Comorbidity; Confidence intervals; Diabetes; Drug therapy; Enzymes; Feasibility Studies; Female; Gene expression; Heart attacks; Heart failure; Heart Failure - diagnosis; Heart Failure - enzymology; Heart Failure - physiopathology; Humans; Hypertension; Logistics; Male; Middle Aged; Natriuretic Peptide, Brain - blood; Patients; Peptides; Peptidyl-Dipeptidase A - blood; Polyclonal antibodies; Regression analysis; Renin-Angiotensin System - physiology; Sensitivity and Specificity; Severity of Illness Index; Statistical analysis; Stroke Volume - physiology; Variance analysis; New York; United States > US
• ISSN: 0735-1097; 1558-3597
• DOI: 10.1016/j.jacc.2008.02.088

We sought to determine whether circulating soluble angiotensin-converting enzyme 2 (sACE2) is increased in the plasma of patients with heart failure (HF). Angiotensin-converting enzyme 2 (ACE2) is an integral membrane protein that antagonizes the actions of angiotensin II and prevents the development of HF in animal models. However, because of the need for invasive cardiac tissue sampling, little is known about whether ACE2 is involved in the pathophysiology of HF in humans. We developed a sensitive and specific assay to measure sACE2 activity in human plasma and screened a heterogeneous group of patients suspected of having clinical HF. Increasing sACE2 plasma activity strongly correlated with a clinical diagnosis of HF (p = 0.0002), worsening left ventricular ejection fraction (p < 0.0001), and increasing B-type natriuretic peptide levels (p < 0.0001). Similar to B-type natriuretic peptide, sACE2 activity reflected the severity of HF, with increasing levels associated with worsening New York Heart Association functional class (p < 0.0001). These associations were independent of other disease states and medication use. We found that sACE2 activity was increased in patients with both ischemic and nonischemic cardiomyopathies and also in patients with clinical HF but a preserved left ventricular ejection fraction. Soluble ACE2 activity is increased in patients with HF and correlates with disease severity, suggesting that a cardioprotective arm of the renin-angiotensin-aldosterone system is active in HF.