Isolation of two chloroethylnitrosourea-sensitive Chinese hamster cell lines

• Published in: Cancer research (Chicago, Ill.) Vol. 51; no. 1; pp. 195 - 198
• Main Authors: HATA, H, NUMATA, M, TOHDA, H, YASUI, A, OIKAWA, A
• Format: Journal Article
• Language: English
• Published: Philadelphia, PA American Association for Cancer Research 1991
• Subjects: 560120 - Radiation Effects on Biochemicals, Cells, & Tissue Culture; 560300 - Chemicals Metabolism & Toxicology; ALKYLATING AGENTS; ANIMAL CELLS; Animals; Antineoplastic agents; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; Cell Line; Cell Survival - drug effects; CHEMICAL REACTIONS; CHO CELLS; Cisplatin - pharmacology; Cricetinae; Cricetulus; CROSS-LINKING; DNA Damage; DNA REPAIR; DNA REPLICATION; Dose-Response Relationship, Drug; DOSE-RESPONSE RELATIONSHIPS; ELECTROMAGNETIC RADIATION; EXCISION REPAIR; General aspects; Genetic Complementation Test; GENETIC EFFECTS; In Vitro Techniques; Medical sciences; Mitomycin; Mitomycins - pharmacology; Nimustine - metabolism; Nimustine - pharmacology; NITROSO COMPOUNDS; NITROSOUREAS; NUCLEIC ACID REPLICATION; ORGANIC COMPOUNDS; ORGANIC NITROGEN COMPOUNDS; Pharmacology. Drug treatments; POLYMERIZATION; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RECOVERY; REPAIR; SENSITIVITY; ULTRAVIOLET RADIATION; Ultraviolet Rays; X-Rays; Cell culture; Nitrosoureas; Rodentia; Crosslinking; In vitro; CHO cell line; Ovary; Vertebrata; Alkylating agent; Sensitivity; Mammalia; Sensitivity resistance; Investigation method; Animal; DNA; Female genital system; Lesion; Repair; Nucleic acid; Hamster
• ISSN: 0008-5472; 1538-7445

1-[(4-Amino-2-methylpyrimidin-5-yl)methyl]-3-(2-chloroethyl)-3- nitrosourea hydrochloride (ACNU), a cancer chemotherapeutic bifunctional alkylating agent, causes chloroethylation of DNA and subsequent DNA strand cross-linking through an ethylene bridge. We isolated and characterized two ACNU-sensitive mutants from mutagenized Chinese hamster ovary cells and found them to be new drug-sensitive recessive Chinese hamster mutants. Both mutants were sensitive to various monofunctional alkylating agents in a way similar to that of the parental cell lines CHO9. One mutant (UVS1) was cross-sensitive to UV and complemented the UV sensitivity of all Chinese hamster cell lines of 7 established complementation groups. Since UV-induced unscheduled DNA synthesis was very low, a new locus related to excision repair is thought to be defective in this cell line. Another ACNU-sensitive mutant, CNU1, was slightly more sensitive to UV than the parent cell line. CNU1 was cross-sensitive to 1-(2-chloroethyl)-3-cyclohexyl-1-nitrosourea and slightly more sensitive to mitomycin C. No increased accumulation of ACNU and a low level of UV-induced unscheduled DNA synthesis in this cell as compared with the parental cell line suggest that there is abnormality in a repair response of this mutant cell to some types of DNA cross-links.