Aberrant Distribution of ADAM3 in Sperm from Both Angiotensin-Converting Enzyme (Ace)- and Calmegin (Clgn)-Deficient Mice
Male mice deficient for the calmegin ( Clgn ) or the angiotensin-converting enzyme ( Ace ) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN is a molecular chaperone for membrane transport of target proteins and ACE is a membran...
Saved in:
Published in | Biology of reproduction Vol. 75; no. 5; pp. 760 - 766 |
---|---|
Main Authors | , , , , |
Format | Journal Article |
Language | English |
Published |
United States
Society for the Study of Reproduction
01.11.2006
|
Subjects | |
Online Access | Get full text |
Cover
Loading…
Summary: | Male mice deficient for the calmegin ( Clgn ) or the angiotensin-converting enzyme ( Ace ) gene show impaired sperm migration into the oviduct and loss of sperm-zona pellucida binding ability in vitro. Since CLGN
is a molecular chaperone for membrane transport of target proteins and ACE is a membrane protein, we looked for ACE on the
sperm membranes from Clgn â/â mice. ACE was present and showed normal activity, indicating that CLGN is not involved in transporting ACE to the sperm membranes.
The ablation of the Adam2 and Adam3 genes generated animals whose sperm did not bind the zona pellucida, which led us to examine the presence of ADAM2 and ADAM3
in Clgn â/â and Ace â/â sperm. ADAM3 was absent from Clgn â/â sperm. In the Ace â/â mice, while ADAM2 was found normally in the sperm, ADAM3 disappeared from the Triton X-114 detergent-enriched phase after
phase separation, which suggests that ACE is involved in distributing ADAM3 to a location where it can participate in sperm-zona
pellucida binding. This diminished amount of ADAM3 in the Triton X-114 detergent-enriched phase may explain the inability
of Clgn â/â and Ace â/â sperm to bind to the zona pellucida.
Abstract
ADAM3 was absent from Clgn â/â sperm and was significantly decreased in the âdetergent-enrichedâ fraction of Ace â/â sperm using Triton X-114, indicating that the ability of sperm to bind the zona pellucida could be explained by the amount
of ADAM3. |
---|---|
Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 |
ISSN: | 0006-3363 1529-7268 |
DOI: | 10.1095/biolreprod.106.052977 |