Detection of the Mr 110,000 Lung Resistance-related Protein LRP/MVP with Monoclonal Antibodies

• Published in: The journal of histochemistry and cytochemistry Vol. 49; no. 11; pp. 1379 - 1385
• Main Authors: Schroeijers, Anouk B, Scheffer, George L, Reurs, Anneke W, Pijnenborg, Adriana C.L.M, Abbondanza, Ciro, Wiemer, Erik A.C, Scheper, Rik J
• Format: Journal Article
• Language: English
• Published: Los Angeles, CA Histochemical Soc 01.11.2001; SAGE Publications
• Subjects: Animals; Antibodies, Monoclonal - biosynthesis; Blotting, Western; Carcinoma, Non-Small-Cell Lung; Carcinoma, Small Cell; Drug Resistance, Multiple; Drug Resistance, Neoplasm; Fixatives; Formaldehyde; Immunohistochemistry; Lung Neoplasms; Male; Mice; Mice, Inbred BALB C; Neoplasm Proteins - immunology; Neoplasm Proteins - metabolism; Paraffin Embedding; Tissue Fixation; Tumor Cells, Cultured; Vault Ribonucleoprotein Particles - immunology; Vault Ribonucleoprotein Particles - metabolism; monoclonal antibody (MAb); major vault protein (MVP); multidrug resistance (MDR); lung resistance-related protein (LRP); vault
• ISSN: 0022-1554; 1551-5044
• DOI: 10.1177/002215540104901106

The Mr 110,000 lung resistance-related protein (LRP), also termed the major vault protein (MVP), constitutes >70% of subcellular ribonucleoprotein particles called vaults. Overexpression of LRP/MVP and vaults has been linked directly to MDR in cancer cells. Clinically, LRP/MVP expression can be of value to predict response to chemotherapy and prognosis. Monoclonal antibodies (MAbs) against LRP/MVP have played a critical role in determining the relevance of this protein in clinical drug resistance. We compared the applicability of the previously described MAbs LRP-56, LMR-5, LRP, 1027, 1032, and newly isolated MAbs MVP-9, MVP-16, MVP-18, and MVP-37 for the immunodetection of LRP/MVP by immunoblotting analysis and by immunocyto- and histochemistry. The availability of a broader panel of reagents for the specific and sensitive immunodetection of LRP/MVP should greatly facilitate biological and clinical studies of vault-related MDR.