Biochemical characterization of the bovine genetic kappa-casein C and E variants

Analysis of elution profiles of enzymatic and CNBr digests of kappa-caseins C and E, and sequencing of most relevant peptides allowed the chemical characterization of both genetic variants. They differ from their B and A allelic counterparts by a single substitution, His97/Arg and Gly155/Ser, respec...

Full description

Saved in:
Bibliographic Details
Published inAnimal genetics Vol. 24; no. 1; p. 27
Main Authors Miranda, G, Anglade, P, Mahé, M F, Erhardt, G
Format Journal Article
LanguageEnglish
Published England 01.02.1993
Subjects
Alleles
Amino Acid Sequence
Animals
Caseins - chemistry
Caseins - genetics
Caseins - isolation & purification
Cattle - genetics
Chromatography, High Pressure Liquid - veterinary
Electrophoresis, Starch Gel - veterinary
Genetic Variation
Isoelectric Focusing - veterinary
Molecular Sequence Data
Peptide Fragments - chemistry
Peptide Fragments - genetics
Peptide Mapping - veterinary
Point Mutation
Sequence Analysis
Sequence Homology, Amino Acid
Online AccessGet more information

Cover

More Information
Summary:Analysis of elution profiles of enzymatic and CNBr digests of kappa-caseins C and E, and sequencing of most relevant peptides allowed the chemical characterization of both genetic variants. They differ from their B and A allelic counterparts by a single substitution, His97/Arg and Gly155/Ser, respectively. Electrophoretic behaviour of the investigated C and E variants was in good agreement with the observed amino acid replacements.
ISSN:0268-9146