Expression of p21WAF1/CIP1 is heterogeneous and unrelated to proliferation index in human ovarian carcinoma

The growth-inhibitory protein p21WAF1/CIP1 is a potent inhibitor of various cyclin-dependent kinases, the expression of which is regulated at the transcriptional level by p53-dependent and -independent mechanisms. We examined p21WAF1/CIP1 mRNA and protein expression in 5 human ovarian-adenocarcinoma...

Full description

Saved in:
Bibliographic Details
Published inInternational journal of cancer Vol. 63; no. 5; p. 611
Main Authors Barboule, N, Mazars, P, Baldin, V, Vidal, S, Jozan, S, Martel, P, Valette, A
Format Journal Article
LanguageEnglish
Published United States 27.11.1995
Subjects
Adenocarcinoma - metabolism
Adenocarcinoma - pathology
Blotting, Western
Cell Division
Cyclin-Dependent Kinase Inhibitor p21
Cyclins - biosynthesis
Female
Humans
Ovarian Neoplasms - metabolism
Ovarian Neoplasms - pathology
RNA, Messenger - metabolism
Tumor Cells, Cultured
Online AccessGet more information

Cover

More Information
Summary:The growth-inhibitory protein p21WAF1/CIP1 is a potent inhibitor of various cyclin-dependent kinases, the expression of which is regulated at the transcriptional level by p53-dependent and -independent mechanisms. We examined p21WAF1/CIP1 mRNA and protein expression in 5 human ovarian-adenocarcinoma cell lines, 1 primary culture of normal surface epithelium and 17 human ovarian-tumor specimens. In culture cells, the p21WAF1/CIP1 protein was expressed in normal ovarian epithelial cells and at a high level in the adenocarcinoma 2008 and IGROV-1 cell lines. p21 WAF1/CIP1 expression was undetectable at the mRNA and protein levels in the NIH-OVCAR-3 and SKOV-3 ovarian-adenocarcinoma cell lines which are respectively mutated and deleted in the p53 gene. Heterogeneous expression of p21WAF1/CIP1 observed in ovarian-cancer cell lines in culture was also found in vivo on tumor specimens. p21WAF1/CIP1 expression is undetectable in 25% of the ovarian biopsies examined. Since it has been found that the p53 gene is mutated in 79% of ovarian cancer, the absence of p21WAF1/CIP1 expression in 25% of these ovarian cancer could not be correlated with p53 mutation. The proliferation index of the 17 tumors showed great variation from one tumor to another. However, no significant correlation was found between p21WAF1/CIP1 expression and the proliferation rate of the tumors.
ISSN:0020-7136
DOI:10.1002/ijc.2910630502