Effects of Porphyromonas gingivalis LPS and LR12 peptide on TREM‐1 expression by monocytes

• Published in: Journal of clinical periodontology Vol. 45; no. 7; pp. 799 - 805
• Main Authors: Dubar, Marie, Carrasco, Kevin, Gibot, Sebastien, Bisson, Catherine
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01.07.2018; Wiley
• Subjects: Cell activation; Chemokines; Cytokines; Dentistry; Enzyme-linked immunosorbent assay; Flow cytometry; Gene expression; Humans; IL-1β; Immune response; Inflammation; Lauric Acids; Life Sciences; Lipopolysaccharides; Monocytes; mRNA; Myeloid cells; P. gingivalis; Periodontics; Porphyromonas gingivalis; primary human monocytes; Rhodamines; TREM‐1; TREM‐1 inhibitory peptide; Triggering Receptor Expressed on Myeloid Cells-1; Tumor necrosis factor-α; TREM-1; cytokines; primary human monocytes; P. gingivalis; TREM-1 inhibitory peptide
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/jcpe.12925

Periodontal disease involves the activation of host immune response, acting not only as defender of periodontal tissues against bacterial aggression but also as mediator of tissue destruction. Triggering receptor expressed on myeloid cells 1 (TREM‐1) is an immune receptor that synergizes with Toll‐like receptors in amplifying the inflammatory response mediated by microbial molecules. Aim To investigate the role of P. gingivalis lipopolysaccharide (LPS) and the effect of LR12, a TREM‐1 inhibitory peptide, on the expression of membrane‐bound and soluble form of TREM‐1 on human primary monocytes, as well as the production of proinflammatory cytokines. Material and Methods Cells were stimulated with 1 μg/ml of LPS with or without LR12. PCR, flow cytometry and ELISA were used to determine TREM‐1 expressions and cytokines release by monocytes. Results P. gingivalis LPS can induce a significant increase in TREM‐1 expression (mRNA, membrane‐bound and soluble form, p < 0.001) as well as cytokines (IL‐1β, TNFα) and chemokines (IL‐8) production by monocytes. This monocytes’ activation was partly prevented by LR12. Conclusions TREM‐1 inhibitors such as LR12 could be interesting for the modulation of the excessive inflammatory response that occurs during periodontal disease.