Engineering of the pyruvate dehydrogenase bypass in Saccharomyces cerevisiae : Role of the cytosolic Mg2+ and mitochondrial K+ acetaldehyde dehydrogenases Ald6p and Ald4p in acetate formation during alcoholic fermentation

Acetic acid plays a crucial role in the organoleptic balance of many fermented products. We have investigated the factors controlling the production of acetate by Saccharomyces cerevisiae during alcoholic fermentation by metabolic engineering of the enzymatic steps involved in its formation and its...

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Published inApplied and environmental microbiology Vol. 66; no. 8; pp. 3151 - 3159
Main Authors REMIZE, F, ANDRIEU, E, DEQUIN, S
Format Journal Article
LanguageEnglish
Published Washington, DC American Society for Microbiology 01.08.2000
Subjects
Acids
Alcohol
Biological and medical sciences
Ecology, environment
Enzymes
Enzymology and Protein Engineering
Fermentation
Fermented food industries
Food industries
Fundamental and applied biological sciences. Psychology
Life Sciences
Microbiology
Mutation
Wines and vinegars
Yeast
Acetate-CoA ligase
Yeast
Enzyme
Organoleptic properties
Carbon-sulfur ligases
Enology
Lyases
Acetate
Metabolism
Fungi
Aldehyde dehydrogenase [NAD-(P)
Carbon-carbon lyases
Enzymatic activity
Alcoholic fermentation
Ligases
Carboxy-lyases
Microorganism culture
Ascomycetes
Genetic engineering
Oxidoreductases
]
Saccharomyces cerevisiae
Thallophyta
Pyruvate decarboxylase
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Summary:Acetic acid plays a crucial role in the organoleptic balance of many fermented products. We have investigated the factors controlling the production of acetate by Saccharomyces cerevisiae during alcoholic fermentation by metabolic engineering of the enzymatic steps involved in its formation and its utilization.
Bibliography:PMCID: PMC92127
Present address: Department of Cell and Molecular Biology/Microbiology, Göteborg University, SE-405 30 Göteborg, Sweden.
Corresponding author. Mailing address: IPV-Laboratoire de Microbiologie et Technologie des Fermentations, INRA, 2 Place Viala F-34060 Montpellier Cedex 2, France. Phone: (33) 4 99 61 25 28. Fax: (33) 4 99 61 28 57. E-mail: dequin@ensam.inra.fr.
ISSN:0099-2240
1098-5336
DOI:10.1128/AEM.66.8.3151-3159.2000